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	Electric-field assisted immobilization and hybridization of DNA oligomers on microcantilever sensors
	Yu, Xiaomei ; Zhang, Haitao ; Li, Xiuham ; Tang, Yaquan ; Li, Ting
	2006
关键词	cantilever immobilization hybridization probe
英文摘要	Gold-coated microcantilevers were used to develop sensitive micromechanical biosensor for detecting mismatch mutations in DNA hybrids. The selective 5'-end immobilization of 25-mer single-stranded (ss) DNA probes to a gold-coated cantilever surface and the specific hybridization of ssDNA targets to the immobilized probe were performed by the direct electric field control. The controlled electric fields were used to regulate the transport of charged oligo-nucleotides. More than 70% of the immobilization and hybridization can be carried out in 2-3 seconds, and the detection rates are at least 102 times faster than in the passive control reactions performed without electric field.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000248485800116&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Engineering, Electrical & Electronic; Nanoscience & Nanotechnology; EI; CPCI-S(ISTP); 0
语种	英语
DOI标识	10.1109/NEMS.2006.334828
内容类型	其他
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/293387]
专题	信息科学技术学院
推荐引用方式 GB/T 7714	Yu, Xiaomei,Zhang, Haitao,Li, Xiuham,et al. Electric-field assisted immobilization and hybridization of DNA oligomers on microcantilever sensors. 2006-01-01.

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