Using optical tweezers to investigate the specific single-interaction   between apoA-I molecule and ABCA1 on living cells

doi:10.3788/COL201311.091701

CORC > 北京大学 > 信息科学技术学院

	Using optical tweezers to investigate the specific single-interaction between apoA-I molecule and ABCA1 on living cells
	Yu, Jie ; Tong, Xunliang ; Li, Chengbin ; Huang, Yining ; Ye, Anpei
刊名	chinese optics letters
	2013
关键词	DYNAMIC FORCE SPECTROSCOPY APOLIPOPROTEIN-A-I BINDING ADHESION CHOLESTEROL STRENGTH INTEGRIN REMOVAL MODELS
DOI	10.3788/COL201311.091701
英文摘要	We carry out in situ single-molecule measurements of the specific interaction between apolipoprotein A-I (apoA-I) and ATP binding cassette transporter A1 (ABCA1) on THP-1 cells. Single-molecule force spectroscopy shows that similar to normal apoA-I, the dysfunctional apoA-I from diabetes patients interacts with ABCA1 via two different binding sites on the cells. The strength of dysfunctional apoA-I binding to a high-capacity binding site is 26.5+/-4.9 pN. The minor direct apoA-I/ABCA1 binding strength is 56.7+/-4.1 pN. These results facilitate a pathological understanding of the mechanisms that underlie the specific interaction of apoA-I and ABCA1 at the single-molecule level.; Optics; SCI(E); EI; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 2; ARTICLE; 9; 11
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/291717]
专题	信息科学技术学院
推荐引用方式 GB/T 7714	Yu, Jie,Tong, Xunliang,Li, Chengbin,et al. Using optical tweezers to investigate the specific single-interaction between apoA-I molecule and ABCA1 on living cells[J]. chinese optics letters,2013.
APA	Yu, Jie,Tong, Xunliang,Li, Chengbin,Huang, Yining,&Ye, Anpei.(2013).Using optical tweezers to investigate the specific single-interaction between apoA-I molecule and ABCA1 on living cells.chinese optics letters.
MLA	Yu, Jie,et al."Using optical tweezers to investigate the specific single-interaction between apoA-I molecule and ABCA1 on living cells".chinese optics letters (2013).