Vertical and horizontal distribution of sediment nitrite-dependent   methane-oxidizing organisms in a mesotrophic freshwater reservoir

doi:10.1139/cjm-2016-0585

CORC > 北京大学 > 环境科学与工程学院

	Vertical and horizontal distribution of sediment nitrite-dependent methane-oxidizing organisms in a mesotrophic freshwater reservoir
	Long, Yan ; Liu, Changbao ; Lin, Hengliang ; Li, Ningning ; Guo, Qingwei ; Xie, Shuguang
刊名	CANADIAN JOURNAL OF MICROBIOLOGY
	2017
关键词	denitrifying methanotrophs bacterial community methane oxidation freshwater sediment 16S RIBOSOMAL-RNA ANAEROBIC OXIDATION PMOA GENES BACTERIA LAKE DIVERSITY WETLAND DENITRIFICATION MICROORGANISMS METHANOTROPHS
DOI	10.1139/cjm-2016-0585
英文摘要	In the present study, we investigated the spatial change of sediment nitrite-dependent anaerobic methane-oxidizing (n-damo) organisms in the mesotrophic freshwater Gaozhou Reservoir (6 different sampling locations and 2 sediment depths (0-5 cm, 5-10 cm)), one of the largest drinking water reservoirs in China. The abundance of sediment n-damo bacteria was quantified using quantitative polymerase chain reaction assay, while the richness, diversity, and composition of n-damo pmoA gene sequences were characterized using clone library analysis. Vertical and horizontal changes in sediment n-damo bacterial abundance occurred in Gaozhou Reservoir, with 1.37 x 10(5) to 8.24 x 10(5) n-damo 16S rRNA gene copies per gram of dry sediment. Considerable horizontal and vertical variations of n-damo pmoA gene diversity (Shannon index = 0.32-2.50) and composition also occurred in this reservoir. Various types of sediment n-damo pmoA genes existed in Gaozhou Reservoir. A small proportion of n-damo pmoA gene sequences (19.1%) were related to those recovered from "Candidatus Methylomirabilis oxyfera". Our results suggested that sediment n-damo pmoA gene diversity might be regulated by nitrite, while n-damo pmoA gene richness might be governed by multiple environmental factors, including total organic carbon, total phosphorus, nitrite, and total nitrogen.; National Natural Science Foundation of China [41001323, 41571444]; SCI(E); ARTICLE; 6; 525-534; 63
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/473114]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Long, Yan,Liu, Changbao,Lin, Hengliang,et al. Vertical and horizontal distribution of sediment nitrite-dependent methane-oxidizing organisms in a mesotrophic freshwater reservoir[J]. CANADIAN JOURNAL OF MICROBIOLOGY,2017.
APA	Long, Yan,Liu, Changbao,Lin, Hengliang,Li, Ningning,Guo, Qingwei,&Xie, Shuguang.(2017).Vertical and horizontal distribution of sediment nitrite-dependent methane-oxidizing organisms in a mesotrophic freshwater reservoir.CANADIAN JOURNAL OF MICROBIOLOGY.
MLA	Long, Yan,et al."Vertical and horizontal distribution of sediment nitrite-dependent methane-oxidizing organisms in a mesotrophic freshwater reservoir".CANADIAN JOURNAL OF MICROBIOLOGY (2017).