Ozonized carbon black induces mitochondrial dysfunction and DNA damage

doi:10.1002/tox.22295

CORC > 北京大学 > 环境科学与工程学院

	Ozonized carbon black induces mitochondrial dysfunction and DNA damage
	Gao, Xin ; Xu, Huadong ; Shang, Jing ; Yuan, Lan ; Zhang, Yongming ; Wang, Lele ; Zhang, Wenxiao ; Luan, Xianguo ; Hu, Guiping ; Chu, Hongqian ; Zhu, Tong ; Jia, Guang
刊名	ENVIRONMENTAL TOXICOLOGY
	2017
关键词	black carbon carbon black ozone mitochondria oxidative stress OXIDATIVE STRESS TITANIUM-DIOXIDE PHYSICOCHEMICAL CHARACTERISTICS AIR-QUALITY PARTICLES NANOPARTICLES OZONE TOXICITY SURFACE CANCER
DOI	10.1002/tox.22295
英文摘要	Black carbon and tropospheric ozone (O-3), which are major air pollutants in China, are hazardous to humans following inhalation. Black carbon can be oxidized by O-3 forming secondary particles of which the health effects are unknown. The present study utilized carbon black as a representative of black carbon to characterize the cytotoxicity induced by secondary particles in bronchial epithelial cells (16HBE) and C57BL/6J mice, and to investigate the implicated molecular pathways. Two types of carbon black including untreated carbon black (UCB) and ozonized carbon black (OCB) were presented. The effects of carbon black on cell viability, intracellular reactive oxygen species (ROS), oxidized/reduced glutathione ratio, mitochondrial membrane potential (MMP), intracellular ATP, and mitochondrial cytochrome c to cytoplasmic cytochrome c ratio were assessed in 16HBE. In addition, an alkaline comet assay and a cytokinesis-block micronucleus (CBMN) test with 16HBE cells in vitro and ELISA method for serum 8-hydroxy-2-deoxyguanosine (8-OHdG) and a bone marrow micronucleus (BMN) test with C57BL/6J mice in vivo were performed to detect the genotoxicity. When compared with UCB exposed cells, OCB exposed cells had decreased cell viability, increased cell death rate, increased comet length and decreased MMP at 24 h exposure. UCB induced higher level of intracellular ROS than OCB from 4 to 23 h. No changes were observed for both OCB and UCB in serum 8-OHdG, intracellular ATP and mitochondrial cytochrome c to cytoplasmic cytochrome c ratio. The results of CBMN and BMN tests are negative. Intracellular ROS induced by OCB was lower than that of UCB. In summary, ozonization enhances the mitochondrial toxicity and genotoxicity of carbon black. Oxidative stress may not dominate in toxic effects of OCB. (C) 2016 Wiley Periodicals, Inc.; Major Program of National Natural Science Foundation of China [21190051]; SCI(E); PubMed; ARTICLE; 3; 944-955; 32
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/433351]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Gao, Xin,Xu, Huadong,Shang, Jing,et al. Ozonized carbon black induces mitochondrial dysfunction and DNA damage[J]. ENVIRONMENTAL TOXICOLOGY,2017.
APA	Gao, Xin.,Xu, Huadong.,Shang, Jing.,Yuan, Lan.,Zhang, Yongming.,...&Jia, Guang.(2017).Ozonized carbon black induces mitochondrial dysfunction and DNA damage.ENVIRONMENTAL TOXICOLOGY.
MLA	Gao, Xin,et al."Ozonized carbon black induces mitochondrial dysfunction and DNA damage".ENVIRONMENTAL TOXICOLOGY (2017).