Variation of nonylphenol-degrading gene abundance and bacterial   community structure in bioaugmented sediment microcosm

doi:10.1007/s11356-014-3625-x

CORC > 北京大学 > 环境科学与工程学院

	Variation of nonylphenol-degrading gene abundance and bacterial community structure in bioaugmented sediment microcosm
	Wang, Zhao ; Yang, Yuyin ; Sun, Weimin ; Dai, Yu ; Xie, Shuguang
刊名	environmental science and pollution research
	2015
关键词	Nonylphenol Bioaugmentation Biodegradation Sediment Stenotrophomonas Sphingobium Microbial community structure Functional gene AMMONIA-OXIDIZING MICROORGANISMS MICROBIAL COMMUNITY RHIZOSPHERE BACTERIA DEGRADATION PATHWAYS PHRAGMITES-AUSTRALIS CONTAMINATED SOIL RIVER SEDIMENT BIODEGRADATION IMPACT IDENTIFICATION
DOI	10.1007/s11356-014-3625-x
英文摘要	Nonylphenol (NP) can accumulate in river sediment. Bioaugmentation is an attractive option to dissipate heavy NP pollution in river sediment. In this study, two NP degraders were isolated from crude oil-polluted soil and river sediment. Microcosms were constructed to test their ability to degrade NP in river sediment. The shift in the proportion of NP-degrading genes and bacterial community structure in sediment microcosms were characterized using quantitative PCR assay and terminal restriction fragment length polymorphism analysis, respectively. Phylogenetic analysis indicated that the soil isolate belonged to genus Stenotrophomonas, while the sediment isolate was a Sphingobium species. Both of them could almost completely clean up a high level of NP in river sediment (150 mg/kg NP) in 10 or 14 days after inoculation. An increase in the proportion of alkB and sMO genes was observed in sediment microcosms inoculated with Stenotrophomonas strain Y1 and Sphingobium strain Y2, respectively. Moreover, bioaugmentation using Sphingobium strain Y2 could have a strong impact on sediment bacterial community structure, while inoculation of Stenotrophomonas strain Y1 illustrated a weak impact. This study can provide some new insights towards NP biodegradation and bioremediation.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000348917400078&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Environmental Sciences; SCI(E); PubMed; 5; ARTICLE; xiesg@pku.edu.cn; 3; 2342-2349; 22
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/158758]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Wang, Zhao,Yang, Yuyin,Sun, Weimin,et al. Variation of nonylphenol-degrading gene abundance and bacterial community structure in bioaugmented sediment microcosm[J]. environmental science and pollution research,2015.
APA	Wang, Zhao,Yang, Yuyin,Sun, Weimin,Dai, Yu,&Xie, Shuguang.(2015).Variation of nonylphenol-degrading gene abundance and bacterial community structure in bioaugmented sediment microcosm.environmental science and pollution research.
MLA	Wang, Zhao,et al."Variation of nonylphenol-degrading gene abundance and bacterial community structure in bioaugmented sediment microcosm".environmental science and pollution research (2015).