Diverse Bacteria with Lignin Degrading Potentials Isolated from Two   Ranks of Coal

doi:10.3389/fmicb.2016.01428

CORC > 北京大学 > 地球与空间科学学院

	Diverse Bacteria with Lignin Degrading Potentials Isolated from Two Ranks of Coal
	Wang, Lu ; Nie, Yong ; Tang, Yue-Qin ; Song, Xin-Min ; Cao, Kun ; Sun, Li-Zhu ; Wang, Zhi-Jian ; Wu, Xiao-Lei
刊名	FRONTIERS IN MICROBIOLOGY
	2016
关键词	lignin degradation coal laccase LMCO gene seed bank SP NOV. MICROBIAL COMMUNITIES RHIZOBIUM SOIL LACCASES GROWTH GENES MINES OIL
DOI	10.3389/fmicb.2016.01428
英文摘要	Taking natural coal as a seed bank of bacterial strains able to degrade lignin that is with molecular structure similar to coal components, we isolated 393 and 483 bacterial strains from a meager lean coal sample from Hancheng coalbed and a brown coal sample from Bayannaoer coalbed, respectively, by using different media. Statistical analysis showed that isolates were significantly more site-specific than medium-specific. Of the 876 strains belonging to 27 genera in Actinobacteria, Firmicutes, and Proteobacteria, 612 were positive for lignin degradation function, including 218 strains belonging to 35 species in Hancheng and 394 strains belonging to 19 species in Zhongqi. Among them, the dominant lignin-degrading strains were Thauera (Hancheng), Arthrobacter (Zhongqi) and Rhizobium (both). The genes encoding the laccases- or laccase-like multicopper oxidases, key enzymes in lignin production and degradation, were detected in three genera including Massila for the first time, which was in high expression by real time PCR (qRT-PCR) detection, confirming coal as a good seed bank.; National Natural Science Foundation of China [31225001, 31300108]; National High Technology Research and Development Program ("863" Program) [2012AA02A703]; National Basic Research Program of China ("973" Program) [2014CB846002]; SCI(E); PubMed; ARTICLE; xiaolei_wu@pku.edu.cn; 1428; 7
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/447987]
专题	地球与空间科学学院工学院
推荐引用方式 GB/T 7714	Wang, Lu,Nie, Yong,Tang, Yue-Qin,et al. Diverse Bacteria with Lignin Degrading Potentials Isolated from Two Ranks of Coal[J]. FRONTIERS IN MICROBIOLOGY,2016.
APA	Wang, Lu.,Nie, Yong.,Tang, Yue-Qin.,Song, Xin-Min.,Cao, Kun.,...&Wu, Xiao-Lei.(2016).Diverse Bacteria with Lignin Degrading Potentials Isolated from Two Ranks of Coal.FRONTIERS IN MICROBIOLOGY.
MLA	Wang, Lu,et al."Diverse Bacteria with Lignin Degrading Potentials Isolated from Two Ranks of Coal".FRONTIERS IN MICROBIOLOGY (2016).