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	Epigenetic regulations of gene expression in human germline cells; Epigenetic regulations of gene expression in human germline cells
	Tang FC(汤富酬)
	2016
关键词	Single cell functional genomics primordial germ cells DNA methylome Single cell sequencing Single cell functional genomics primordial germ cells DNA methylome Single cell sequencing
英文摘要	Measuring gene expression in individual cells is crucial for understanding the gene regulatory network controlling human embryonic development. We applied single-cell RNA-Seq analysis to human preimplantation embryos, primordial germ cells(PGCs), and human embryonic stem cells(h ESCs). We also systematically profiled the DNA methylome of human early embryos from the zygotic stage through to post-implantation. We showed that the major wave of genome-wide demethylation is complete at the 2-cell stage, contrary to previous observations in mice. Moreover, the demethylation of the paternal genome was much faster than that of the maternal genome, and by the end of the zygotic stage the genome-wide methylation level in male pronuclei was already lower than that in female pronuclei. Then we also showed that long interspersed nuclear elements(LINEs) or short interspersed nuclear elements(SINEs) that were evolutionarily young are demethylated to a milder extent compared to older elements in the same family and had high...; Measuring gene expression in individual cells is crucial for understanding the gene regulatory network controlling human embryonic development. We applied single-cell RNA-Seq analysis to human preimplantation embryos, primordial germ cells(PGCs), and human embryonic stem cells(h ESCs). We also systematically profiled the DNA methylome of human early embryos from the zygotic stage through to post-implantation. We showed that the major wave of genome-wide demethylation is complete at the 2-cell stage, contra; 中国生物化学与分子生物学会; 1
语种	英语
出处	中国生物化学与分子生物学会2016年全国学术会议
内容类型	其他
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/479834]
专题	生命科学学院
推荐引用方式 GB/T 7714	Tang FC. Epigenetic regulations of gene expression in human germline cells, Epigenetic regulations of gene expression in human germline cells. 2016-01-01.

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