Centrifugal micro-channel array droplet generation for highly parallel   digital PCR

doi:10.1039/c6lc01305h

CORC > 北京大学 > 生命科学学院

	Centrifugal micro-channel array droplet generation for highly parallel digital PCR
	Chen, Zitian ; Liao, Peiyu ; Zhang, Fangli ; Jiang, Mengcheng ; Zhu, Yusen ; Huang, Yanyi
刊名	LAB ON A CHIP
	2017
关键词	POLYMERASE-CHAIN-REACTION DNA COPY NUMBER TIME QUANTITATIVE PCR RT-PCR ABSOLUTE QUANTIFICATION MICRODROPLETS AMPLIFICATION ANEUPLOIDY DEVICE
DOI	10.1039/c6lc01305h
英文摘要	Stable water-in-oil emulsion is essential to digital PCR and many other bioanalytical reactions that employ droplets as microreactors. We developed a novel technology to produce monodisperse emulsion droplets with high efficiency and high throughput using a bench-top centrifuge. Upon centrifugal spinning, the continuous aqueous phase is dispersed into monodisperse droplet jets in air through a micro-channel array (MiCA) and then submerged into oil as a stable emulsion. We performed dPCR reactions with a high dynamic range through the MiCA approach, and demonstrated that this cost-effective method not only eliminates the usage of complex microfluidic devices and control systems, but also greatly suppresses the loss of materials and cross-contamination. MiCA-enabled highly parallel emulsion generation combines both easiness and robustness of picoliter droplet production, and breaks the technical challenges by using conventional lab equipment and supplies.; Ministry of Science and Technology of China [2015AA0200601, 2016YFC0900100]; National Natural Science Foundation of China [21327808, 21525521]; SCI(E); ARTICLE; 2; 235-240; 17
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/475625]
专题	生命科学学院工学院
推荐引用方式 GB/T 7714	Chen, Zitian,Liao, Peiyu,Zhang, Fangli,et al. Centrifugal micro-channel array droplet generation for highly parallel digital PCR[J]. LAB ON A CHIP,2017.
APA	Chen, Zitian,Liao, Peiyu,Zhang, Fangli,Jiang, Mengcheng,Zhu, Yusen,&Huang, Yanyi.(2017).Centrifugal micro-channel array droplet generation for highly parallel digital PCR.LAB ON A CHIP.
MLA	Chen, Zitian,et al."Centrifugal micro-channel array droplet generation for highly parallel digital PCR".LAB ON A CHIP (2017).