The precise regulation of different COR genes by individual CBF   transcription factors in Arabidopsis thaliana

doi:10.1111/jipb.12515

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	The precise regulation of different COR genes by individual CBF transcription factors in Arabidopsis thaliana
	Shi, Yihao ; Huang, Jiaying ; Sun, Tianshu ; Wang, Xuefei ; Zhu, Chenqi ; Ai, Yuxi ; Gu, Hongya
刊名	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
	2017
关键词	CRISPR Cas9 CBF cold tolerance COR single CBF-regulated genes CRISPR-CAS SYSTEM FREEZING TOLERANCE COLD-ACCLIMATION NATURAL VARIATION LOW-TEMPERATURE EXPRESSION ANALYSIS PATHWAY PLANTS ACTIVATOR GENETICS
DOI	10.1111/jipb.12515
英文摘要	The transcription factors CBF1/2/3 are reported to play a dominant role in the cold responsive network of Arabidopsis by directly regulating the expression levels of cold responsive (COR) genes. In this study, we obtained CRISPR/Cas9-mediated loss-of-function mutants of cbf1 approximate to 3. Over 3,000 COR genes identified by RNA-seq analysis showed a slight but significant change in their expression levels in the mutants compared to the wild-type plants after being treated at 4 degrees C for 12h. The C-repeat (CRT) motif (5-CCGAC-3) was enriched in promoters of genes that were up-regulated by CBF2 and CBF3 but not in promoters of genes up-regulated by CBF1. These data suggest that CBF2 and CBF3 play a more important role in directing the cold response by regulating different sets of downstream COR genes. More than 2/3 of COR genes were co-regulated by two or three CBFs and were involved mainly in cellular signal transduction and metabolic processes; less than 1/3 of the genes were regulated by one CBF, and those genes up-regulated were enriched in cold-related abiotic stress responses. Our results indicate that CBFs play an important role in the trade-off between cold tolerance and plant growth through the precise regulation of COR genes in the complicated transcriptional network.; National Natural Science Foundation of China [91331201]; Ministry of Agriculture of China [2014ZX08009-001]; SCI(E); 中国科学引文数据库(CSCD); ARTICLE; 2; 118-133; 59
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/475315]
专题	生命科学学院
推荐引用方式 GB/T 7714	Shi, Yihao,Huang, Jiaying,Sun, Tianshu,et al. The precise regulation of different COR genes by individual CBF transcription factors in Arabidopsis thaliana[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2017.
APA	Shi, Yihao.,Huang, Jiaying.,Sun, Tianshu.,Wang, Xuefei.,Zhu, Chenqi.,...&Gu, Hongya.(2017).The precise regulation of different COR genes by individual CBF transcription factors in Arabidopsis thaliana.JOURNAL OF INTEGRATIVE PLANT BIOLOGY.
MLA	Shi, Yihao,et al."The precise regulation of different COR genes by individual CBF transcription factors in Arabidopsis thaliana".JOURNAL OF INTEGRATIVE PLANT BIOLOGY (2017).