Engineering Escherichia coli to bind to cyanobacteria

doi:10.1016/jpiosc.2016.09.010

CORC > 北京大学 > 生命科学学院

	Engineering Escherichia coli to bind to cyanobacteria
	Zhang, Zijian ; Meng, Liuyi ; Ni, Congjian ; Yao, Lanqiu ; Zhang, Fengyu ; Jin, Yuji ; Mu, Xuelang ; Zhu, Shiyu ; Lu, Xiaoyu ; Liu, Shiyu ; Yu, Congyu ; Wang, Chenggong ; Zheng, Pu ; Wu, Jie ; Kang, Li ; Zhang, Haoqian M. ; Ouyang, Qi
刊名	JOURNAL OF BIOSCIENCE AND BIOENGINEERING
	2017
关键词	Surface display Lectin Microvirin Binding Cyanobacteria Escherichia coil LECTIN MICROCYSTINS NUTRITION
DOI	10.1016/jpiosc.2016.09.010
英文摘要	We engineered Escherichia coli cells to bind to cyanobacteria by heterologously producing and displaying lectins of the target cyanobacteria on their surface. To prove the efficacy of our approach, we tested this design on Micro cystis aeruginosa with microvirin (Mvn), the lectin endogenously produced by this cyanobacterium. The coding sequence of Mvn was C-terminally fused to the ice nucleation protein NC (INPNC) gene and expressed in E. coll. Results showed that E. colt cells expressing the INPNC::Mvn fusion protein were able to bind to M. aeruginosa and the average number of E. colt cells bound to each cyanobacterial cell was enhanced 8-fold. Finally, a computational model was developed to simulate the binding reaction and help reconstruct the binding parameters. To our best knowledge, this is the first report on the binding of two organisms in liquid culture mediated by the surface display of lectins and it may serve as a novel approach to mediate microbial adhesion. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.; Peking University; Fund for Fostering Talents in Basic Science of the National Natural Science Foundation of China [J1310033]; SCI(E); ARTICLE; 3; 347-352; 123
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/474853]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhang, Zijian,Meng, Liuyi,Ni, Congjian,et al. Engineering Escherichia coli to bind to cyanobacteria[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2017.
APA	Zhang, Zijian.,Meng, Liuyi.,Ni, Congjian.,Yao, Lanqiu.,Zhang, Fengyu.,...&Ouyang, Qi.(2017).Engineering Escherichia coli to bind to cyanobacteria.JOURNAL OF BIOSCIENCE AND BIOENGINEERING.
MLA	Zhang, Zijian,et al."Engineering Escherichia coli to bind to cyanobacteria".JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2017).