Expression Analyses of Embryogenesis-Associated Genes during Somatic   Embryogenesis of Adiantum capillus-veneris L. In vitro: New Insights   into the Evolution of Reproductive Organs in Land Plants

doi:10.3389/fpls.2017.00658

CORC > 北京大学 > 生命科学学院

	Expression Analyses of Embryogenesis-Associated Genes during Somatic Embryogenesis of Adiantum capillus-veneris L. In vitro: New Insights into the Evolution of Reproductive Organs in Land Plants
	Li, Xia ; Han, Jing-Dan ; Fang, Yu-Han ; Bai, Shu-Nong ; Rao, Guang-Yuan
刊名	FRONTIERS IN PLANT SCIENCE
	2017
关键词	Adiantum capillus-veneris somatic embryogenesis embryogenesis-associated genes gene expression phylogenetic analysis TRANSCRIPTION FACTOR FAMILY PATTERN-FORMATION CALLUS-CULTURE REGENERATION ARABIDOPSIS FERN ORIGIN ORGANOGENESIS SEED DIVERSIFICATION
DOI	10.3389/fpls.2017.00658
英文摘要	An efficient in vitro regeneration system via somatic embryogenesis (SE) was developed for a fern species Adiantum capillus-veneris. Adventitious shoots, green globular bodies (GGBs) and calli were obtained with the maximal induction rate on the Murashige and Skoog (MS) medium of low concentrations of 6-benzyladenine (BA) (0-1.0 mg/L), 2.0mg/L BA without 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5 mg/L 2,4-D and 0.5-1.0 mg/L 6-BA, respectively. Cyto-morphological and histological changes in the shoot development via calli and GGBs were examined. For a better understanding of these developmental events, expression patterns of six genes, AcLBD16, AcAGL, AcBBM, AcWUS, AcRKD, and AcLEC1, were characterized during SE. AcBBM and AcLEC1 were ubiquitously expressed in direct SE (adventitious shoots and GGBs) the maximal expression of AcBBM in mature GGBs, and the high expression of AcLEC1 in GGB initiation and adventitious shoots. During the indirect SE, AcLBD16, AcLEC1, AcRKD, and AcWUS were highly expressed in mature calli. Additionally, phylogenetic analyses showed that AcWUS, AcBBM, AcLBD, AcAGL, AcRKD, and their homologs of other green plants formed monophyletic clades, respectively. Some of these gene families, however, diversified rapidly with the occurrence of embryophytes, suggesting that embryogenesis-associated genes could experience a rapid evolution with the colonization of plants to terrestrial environments. Expression and phylogenetic analyses of those embryogenesis-associated genes by the aid of in vitro regeneration system of A. capillus-veneris provide new insights into the evolution of reproductive organs in land plants.; National Natural Science Foundation of China (NSFC) [91231105]; SCI(E); ARTICLE; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/473874]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, Xia,Han, Jing-Dan,Fang, Yu-Han,et al. Expression Analyses of Embryogenesis-Associated Genes during Somatic Embryogenesis of Adiantum capillus-veneris L. In vitro: New Insights into the Evolution of Reproductive Organs in Land Plants[J]. FRONTIERS IN PLANT SCIENCE,2017.
APA	Li, Xia,Han, Jing-Dan,Fang, Yu-Han,Bai, Shu-Nong,&Rao, Guang-Yuan.(2017).Expression Analyses of Embryogenesis-Associated Genes during Somatic Embryogenesis of Adiantum capillus-veneris L. In vitro: New Insights into the Evolution of Reproductive Organs in Land Plants.FRONTIERS IN PLANT SCIENCE.
MLA	Li, Xia,et al."Expression Analyses of Embryogenesis-Associated Genes during Somatic Embryogenesis of Adiantum capillus-veneris L. In vitro: New Insights into the Evolution of Reproductive Organs in Land Plants".FRONTIERS IN PLANT SCIENCE (2017).