Landscape of Infiltrating T Cells in Liver Cancer Revealed by   Single-Cell Sequencing

doi:10.1016/j.cell.2017.05.035

CORC > 北京大学 > 生命科学学院

	Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing
	Zheng, Chunhong ; Zheng, Liangtao ; Yoo, Jae-Kwang ; Guo, Huahu ; Zhang, Yuanyuan ; Guo, Xinyi ; Kang, Boxi ; Hu, Ruozhen ; Huang, Julie Y. ; Zhang, Qiming ; Liu, Zhouzerui ; Dong, Minghui ; Hu, Xueda ; Ouyang, Wenjun ; Peng, Jirun ; Zhang, Zemin
刊名	CELL
	2017
关键词	IMMUNE CHECKPOINT BLOCKADE RNA-SEQ ANALYSIS HEPATOCELLULAR-CARCINOMA MAIT CELLS IMMUNOTHERAPY LYMPHOCYTES EXHAUSTION THERAPY IMMUNOPATHOGENESIS EXPRESSION
DOI	10.1016/j.cell.2017.05.035
英文摘要	Systematic interrogation of tumor-infiltrating lymphocytes is key to the development of immunotherapies and the prediction of their clinical responses in cancers. Here, we perform deep single-cell RNA sequencing on 5,063 single T cells isolated from peripheral blood, tumor, and adjacent normal tissues from six hepatocellular carcinoma patients. The transcriptional profiles of these individual cells, coupled with assembled T cell receptor (TCR) sequences, enable us to identify 11 T cell subsets based on their molecular and functional properties and delineate their developmental trajectory. Specific subsets such as exhausted CD8(+) T cells and Tregs are preferentially enriched and potentially clonally expanded in hepatocellular carcinoma (HCC), and we identified signature genes for each subset. One of the genes, layilin, is upregulated on activated CD8(+) T cells and Tregs and represses the CD8(+) T cell functions in vitro. This compendium of transcriptome data provides valuable insights and a rich resource for understanding the immune landscape in cancers.; Beijing Advanced Innovation Center for Genomics at Peking University; Key Technologies RD Program [2016YFC0900100]; National Natural Science Foundation of China [81573022, 31530036, 81372604]; Postdoctoral Fellowship of Peking-Tsinghua Center for Life Sciences; SCI(E); ARTICLE; 7; 1342-+; 169
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/472788]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zheng, Chunhong,Zheng, Liangtao,Yoo, Jae-Kwang,et al. Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing[J]. CELL,2017.
APA	Zheng, Chunhong.,Zheng, Liangtao.,Yoo, Jae-Kwang.,Guo, Huahu.,Zhang, Yuanyuan.,...&Zhang, Zemin.(2017).Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing.CELL.
MLA	Zheng, Chunhong,et al."Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing".CELL (2017).