CDK4 protein is degraded by anaphase-promoting complex/cyclosome in   mitosis and reaccumulates in early G(1) phase to initiate a new cell   cycle in HeLa cells

doi:10.1074/jbc.M116.773226

CORC > 北京大学 > 生命科学学院

	CDK4 protein is degraded by anaphase-promoting complex/cyclosome in mitosis and reaccumulates in early G(1) phase to initiate a new cell cycle in HeLa cells
	Chen, Huabo ; Xu, Xiaowei ; Wang, Guopeng ; Zhang, Boyan ; Wang, Gang ; Xin, Guangwei ; Liu, Junjun ; Jiang, Qing ; Zhang, Hongyin ; Zhang, Chuanmao
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2017
关键词	DEPENDENT KINASE SUBCELLULAR-LOCALIZATION NUCLEAR ACCUMULATION D1 DEGRADATION CANCER CELLS EXPRESSION PHOSPHORYLATION PROLIFERATION INHIBITION PATHWAY
DOI	10.1074/jbc.M116.773226
英文摘要	CDK4 regulates G(1)/S phase transition in the mammalian cell cycle by phosphorylating retinoblastoma family proteins. However, the mechanism underlying the regulation of CDK4 activity is not fully understood. Here, we show that CDK4 protein is degraded by anaphase-promoting complex/ cyclosome (APC/C) during metaphase-anaphase transition in HeLa cells, whereas its main regulator, cyclin D1, remains intact but is sequestered in cytoplasm. CDK4 protein reaccumulates in the following G(1) phase and shuttles between the nucleus and the cytoplasm to facilitate the nuclear import of cyclin D1. Without CDK4, cyclin D1 cannot enter the nucleus. Point mutations that disrupt CDK4 and cyclin D1 interaction impair the nuclear import of cyclin D1 and the activity of CDK4. RNAi knockdown of CDK4 also induces cytoplasmic retention of cyclin D1 and G(0)/G(1) phase arrest of the cells. Collectively, our data demonstrate that CDK4 protein is degraded in late mitosis and reaccumulates in the following G(1) phase to facilitate the nuclear import of cyclin D1 for activation of CKD4 to initiate a new cell cycle in HeLa cells.; National Natural Science Foundation of China (NSFC) [31371365, 31520103906, 31430051]; Ministry of Science and Technology of China [2016YFA0100501, 2016YFA0500201]; SCI(E); ARTICLE; 24; 10131-10141; 292
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/472769]
专题	生命科学学院
推荐引用方式 GB/T 7714	Chen, Huabo,Xu, Xiaowei,Wang, Guopeng,et al. CDK4 protein is degraded by anaphase-promoting complex/cyclosome in mitosis and reaccumulates in early G(1) phase to initiate a new cell cycle in HeLa cells[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2017.
APA	Chen, Huabo.,Xu, Xiaowei.,Wang, Guopeng.,Zhang, Boyan.,Wang, Gang.,...&Zhang, Chuanmao.(2017).CDK4 protein is degraded by anaphase-promoting complex/cyclosome in mitosis and reaccumulates in early G(1) phase to initiate a new cell cycle in HeLa cells.JOURNAL OF BIOLOGICAL CHEMISTRY.
MLA	Chen, Huabo,et al."CDK4 protein is degraded by anaphase-promoting complex/cyclosome in mitosis and reaccumulates in early G(1) phase to initiate a new cell cycle in HeLa cells".JOURNAL OF BIOLOGICAL CHEMISTRY (2017).