Digital PCR: Endless Frontier of &apos;Divide and Conquer&apos;

doi:10.3390/mi8080231

CORC > 北京大学 > 生命科学学院

	Digital PCR: Endless Frontier of 'Divide and Conquer'
	Liao, Peiyu ; Huang, Yanyi
刊名	MICROMACHINES
	2017
关键词	digital polymerase chain reaction (PCR) microfluidics emulsion droplet microwell chip POLYMERASE-CHAIN-REACTION PICOLITER DROPLETS ARRAY QUANTITATION SYSTEM
DOI	10.3390/mi8080231
英文摘要	Digital polymerase chain reaction (PCR) is becoming ever more recognized amid the overwhelming revolution in DNA quantification, genomics, genetics, and diagnostics led by technologies such as next generation sequencing and studies at the single-cell level. The demand to quantify the amount of DNA and RNA has been driven to the molecular level and digital PCR, with its unprecedented quantification capability, is sure to shine in the coming era. Two decades ago, it emerged as a concept; yet one decade ago, integration with microfluidics invigorated this field. Today, many methods have come to public knowledge and applications surrounding digital PCR is mounting. However, to reach wider accessibility and better practicality, efforts are needed to tackle the remaining problems. This perspective looks back at several inspiring and influential digital PCR approaches in the past and tries to provide a futuristic picture of the trends of digital PCR technologies to come.; National Natural Science Foundation of China [21327808, 21525521]; Ministry of Science and Technology of China [2015AA0200601, 2016YFC0900100]; Beijing Advanced Innovation Center for Genomics; SCI(E); ARTICLE; 8; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/471674]
专题	生命科学学院工学院
推荐引用方式 GB/T 7714	Liao, Peiyu,Huang, Yanyi. Digital PCR: Endless Frontier of 'Divide and Conquer'[J]. MICROMACHINES,2017.
APA	Liao, Peiyu,&Huang, Yanyi.(2017).Digital PCR: Endless Frontier of 'Divide and Conquer'.MICROMACHINES.
MLA	Liao, Peiyu,et al."Digital PCR: Endless Frontier of 'Divide and Conquer'".MICROMACHINES (2017).