Cryo-EM structures of the 80S ribosomes from human parasites Trichomonas   vaginalis and Toxoplasma gondii

doi:10.1038/cr.2017.104

CORC > 北京大学 > 生命科学学院

	Cryo-EM structures of the 80S ribosomes from human parasites Trichomonas vaginalis and Toxoplasma gondii
	Li, Zhifei ; Guo, Qiang ; Zheng, Lvqin ; Ji, Yongsheng ; Xie, Yi-Ting ; Lai, De-Hua ; Lun, Zhao-Rong ; Suo, Xun ; Gao, Ning
刊名	CELL RESEARCH
	2017
关键词	cryo-electron microscopy ribosome translation Trichomonas vaginalis Toxoplasma gondii RNA TRANSLATION INITIATION COMPLEX PEPTIDYL-TRANSFERASE CENTER NEIGHBOR-JOINING METHOD N-TERMINAL EXTENSION EUKARYOTIC RIBOSOME MITOCHONDRIAL RIBOSOME 70S RIBOSOME ANGSTROM RESOLUTION ELECTRON-MICROSCOPY PROTEIN-SYNTHESIS
DOI	10.1038/cr.2017.104
英文摘要	As an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. High-resolution structures of pathogen ribosomes are crucial for understanding the general and unique aspects of translation control in disease-causing microbes. With cryo-electron microscopy technique, we have determined structures of the cytosolic ribosomes from two human parasites, Trichomonas vaginalis and Toxoplasma gondii, at resolution of 3.2-3.4 angstrom. Although the ribosomal proteins from both pathogens are typical members of eukaryotic families, with a co-evolution pattern between certain species-specific insertions/extensions and neighboring ribosomal RNA (rRNA) expansion segments, the sizes of their rRNAs are sharply different. Very interestingly, rRNAs of T. vaginalis are in size comparable to prokaryotic counterparts, with nearly all the eukaryote-specific rRNA expansion segments missing. These structures facilitate the dissection of evolution path for ribosomal proteins and RNAs, and may aid in design of novel translation inhibitors.; National Natural Science Foundation of China [31630087, 31422016, 31470722, 31472058, 31672276]; Special Fund for Agro-scientific Research in the Public Interest [201303042]; SCI(E); ARTICLE; 10; 1275-1288; 27
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/470560]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, Zhifei,Guo, Qiang,Zheng, Lvqin,et al. Cryo-EM structures of the 80S ribosomes from human parasites Trichomonas vaginalis and Toxoplasma gondii[J]. CELL RESEARCH,2017.
APA	Li, Zhifei.,Guo, Qiang.,Zheng, Lvqin.,Ji, Yongsheng.,Xie, Yi-Ting.,...&Gao, Ning.(2017).Cryo-EM structures of the 80S ribosomes from human parasites Trichomonas vaginalis and Toxoplasma gondii.CELL RESEARCH.
MLA	Li, Zhifei,et al."Cryo-EM structures of the 80S ribosomes from human parasites Trichomonas vaginalis and Toxoplasma gondii".CELL RESEARCH (2017).