The Arabidopsis Anaphase-Promoting Complex/Cyclosome Subunit 1 is Critical for Both Female Gametogenesis and Embryogenesis

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	The Arabidopsis Anaphase-Promoting Complex/Cyclosome Subunit 1 is Critical for Both Female Gametogenesis and Embryogenesis
	Wang Yanbing ; Hou Yingnan ; Gu Hongya ; Kang Dingming ; Chen ZhangLiang ; Liu Jingjing ; Qu LiJia
刊名	Journal of Integrative Plant Biology
	2013
关键词	Arabidopsis anaphase-promoting complex/cyclosome (APC/C) APC1 APC4 female gametogenesis embryogenesis synergistic role
英文摘要	Anaphase-promoting complex/cyclosome (APC/C), a multisubunit E3 ligase, plays a critical role in cell cycle control, but the functional characterization of each subunit has not yet been completed. To investigate the function of APC1 in Arabidopsis, we analyzed four mutant alleles of APC1, and found that mutation in APC1 resulted in significantly reduced plant fertility, accumulation of cyclin B, and disrupted auxin distribution in embryos. The three mutant alleles apc1–1, apc1–2 and apc1–3 shared variable defects in female gametogenesis including degradation, abnormal nuclear number, and disrupted polarity of nuclei in the embryo sac as well as in embryogenesis, in which embryos were arrested at multiple stages. All of these defects are similar to those previously identified in apc4. The mutant apc1–4, in which the T-DNA was inserted after the transmembrane domain at the C-terminus, showed much more severe phenotypes; that is, most of the ovules were arrested at the one-nucleate female gametophyte stage (stage FG1). In the apc1 apc4 double mutants, the fertility was further reduced by one-third in apc1–1/+ apc4–1/+, and in some cases no ovules even survived in siliques of apc1–4/+ apc4–1/+. Our data thus suggest that APC1, an essential component of APC/C, plays a synergistic role with APC4 both in female gametogenesis and in embryogenesis.; supported by the National Key Basic Research Program of the People’s Republic of China,the National Science Foundation of China; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 1; 64-74; 55
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/462138]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang Yanbing,Hou Yingnan,Gu Hongya,et al. The Arabidopsis Anaphase-Promoting Complex/Cyclosome Subunit 1 is Critical for Both Female Gametogenesis and Embryogenesis[J]. Journal of Integrative Plant Biology,2013.
APA	Wang Yanbing.,Hou Yingnan.,Gu Hongya.,Kang Dingming.,Chen ZhangLiang.,...&Qu LiJia.(2013).The Arabidopsis Anaphase-Promoting Complex/Cyclosome Subunit 1 is Critical for Both Female Gametogenesis and Embryogenesis.Journal of Integrative Plant Biology.
MLA	Wang Yanbing,et al."The Arabidopsis Anaphase-Promoting Complex/Cyclosome Subunit 1 is Critical for Both Female Gametogenesis and Embryogenesis".Journal of Integrative Plant Biology (2013).