Development and Applications of the Copper-Catalyzed Azide-Alkyne   Cycloaddition (CuAAC) as a Bioorthogonal Reaction

doi:10.3390/molecules21101393

CORC > 北京大学 > 生命科学学院

	Development and Applications of the Copper-Catalyzed Azide-Alkyne Cycloaddition (CuAAC) as a Bioorthogonal Reaction
	Li, Li ; Zhang, Zhiyuan
刊名	MOLECULES
	2016
关键词	CuAAC click reaction bioorthogonal reactions imaging activity-based protein profiling FREE CLICK CHEMISTRY SITE-SPECIFIC INCORPORATION CELL-SURFACE GLYCANS IN-VIVO LIVING CELLS 1,3-DIPOLAR CYCLOADDITIONS O-GLCNAC SACCHAROMYCES-CEREVISIAE FLUORESCENT PROTEIN CHELATING AZIDES
DOI	10.3390/molecules21101393
英文摘要	The emergence of bioorthogonal reactions has greatly broadened the scope of biomolecule labeling and detecting. Of all the bioorthogonal reactions that have been developed, the copper-catalyzed azide-alkyne cycloaddition (CuAAC) is the most widely applied one, mainly because of its relatively fast kinetics and high efficiency. However, the introduction of copper species to in vivo systems raises the issue of potential toxicity. In order to reduce the copper-induced toxicity and further improve the reaction kinetics and efficiency, different strategies have been adopted, including the development of diverse copper chelating ligands to assist the catalytic cycle and the development of chelating azides as reagents. Up to now, the optimization of CuAAC has facilitated its applications in labeling and identifying either specific biomolecule species or on the omics level. Herein, we mainly discuss the efforts in the development of CuAAC to better fit the bioorthogonal reaction criteria and its bioorthogonal applications both in vivo and in vitro.; National High Technology Project [2011CB504300]; SCI(E); PubMed; REVIEW; lili0224@nibs.ac.cn; zhangzhiyuan@nibs.ac.cn; 10; 21
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/457251]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, Li,Zhang, Zhiyuan. Development and Applications of the Copper-Catalyzed Azide-Alkyne Cycloaddition (CuAAC) as a Bioorthogonal Reaction[J]. MOLECULES,2016.
APA	Li, Li,&Zhang, Zhiyuan.(2016).Development and Applications of the Copper-Catalyzed Azide-Alkyne Cycloaddition (CuAAC) as a Bioorthogonal Reaction.MOLECULES.
MLA	Li, Li,et al."Development and Applications of the Copper-Catalyzed Azide-Alkyne Cycloaddition (CuAAC) as a Bioorthogonal Reaction".MOLECULES (2016).