Genome-scale deletion screening of human long non-coding RNAs using a   paired-guide RNA CRISPR-Cas9 library

doi:10.1038/nbt.3715

CORC > 北京大学 > 生命科学学院

	Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library
	Zhu, Shiyou ; Li, Wei ; Liu, Jingze ; Chen, Chen-Hao ; Liao, Qi ; Xu, Ping ; Xu, Han ; Xiao, Tengfei ; Cao, Zhongzheng ; Peng, Jingyu ; Yuan, Pengfei ; Brown, Myles ; Liu, Xiaole Shirley ; Wei, Wensheng
刊名	NATURE BIOTECHNOLOGY
	2016
关键词	HUMAN-CELLS FUNCTIONAL GENOMICS CRISPR/CAS9 LIBRARY ESSENTIAL GENES ACTIVATION SYSTEM DNA IDENTIFICATION REPRESSION CANCER
DOI	10.1038/nbt.3715
英文摘要	CRISPR-Cas9 screens have been widely adopted to analyze coding-gene functions, but high-throughput screening of non-coding elements using this method is more challenging because indels caused by a single cut in non-coding regions are unlikely to produce a functional knockout. A high-throughput method to produce deletions of non-coding DNA is needed. We report a high throughput genomic deletion strategy to screen for functional long non-coding RNAs (IncRNAs) that is based on a lentiviral paired-guide RNA (pgRNA) library. Applying our screening method, we identified 51 IncRNAs that can positively or negatively regulate human cancer cell growth. We validated 9 of 51 IncRNA hits using CRISPR-Cas9-mediated genomic deletion, functional rescue, CRISPR activation or inhibition and gene-expression profiling. Our high-throughput pgRNA genome deletion method will enable rapid identification of functional mammalian non-coding elements.; National Science Foundation of China [NSFC31430025, NSFC31170126, NSFC81471909]; Peking-Tsinghua Center for Life Sciences; NIH grant [U01 CA180980, R01 HG008728]; Claudia Adams Barr Award in Innovative Basic Cancer Research from the Dana-Farber Cancer Institute; Beijing Advanced Innovation Center for Genomics at Peking University; SCI(E); PubMed; ARTICLE; xsliu.dfci@gmail.com; wswei@pku.edu.cn; 12; 1279-1286; 34
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/457183]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhu, Shiyou,Li, Wei,Liu, Jingze,et al. Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library[J]. NATURE BIOTECHNOLOGY,2016.
APA	Zhu, Shiyou.,Li, Wei.,Liu, Jingze.,Chen, Chen-Hao.,Liao, Qi.,...&Wei, Wensheng.(2016).Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library.NATURE BIOTECHNOLOGY.
MLA	Zhu, Shiyou,et al."Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library".NATURE BIOTECHNOLOGY (2016).