Atg20-and Atg24-family proteins promote organelle autophagy in fission   yeast

doi:10.1242/jcs.194373

CORC > 北京大学 > 生命科学学院

	Atg20-and Atg24-family proteins promote organelle autophagy in fission yeast
	Zhao, Dan ; Liu, Xiao-Man ; Yu, Zhong-Qiu ; Sun, Ling-Ling ; Xiong, Xingchuang ; Dong, Meng-Qiu ; Du, Li-Lin
刊名	JOURNAL OF CELL SCIENCE
	2016
关键词	Autophagy Autophagosome Atg20 Atg24 PX-BAR Fission yeast Schizosaccharomyces pombe VACUOLE TARGETING PATHWAY IN-VITRO RECONSTITUTION SELECTIVE AUTOPHAGY SCHIZOSACCHAROMYCES-POMBE SACCHAROMYCES-CEREVISIAE NITROGEN STARVATION CARBOXYPEPTIDASE-Y TAGS PEROXISOMES MOLECULAR-BASIS RECEPTOR
DOI	10.1242/jcs.194373
英文摘要	Autophagy cargos include not only soluble cytosolic materials but also bulky organelles, such as ER and mitochondria. In budding yeast, two proteins that contain the PX domain and the BAR domain, Atg20 and Atg24 (also known as Snx42 and Snx4, respectively) are required for organelle autophagy and contribute to general autophagy in a way that can be masked by compensatory mechanisms. It remains unclear why these proteins are important for organelle autophagy. Here, we show that in a distantly related fungal organism, the fission yeast Schizosaccharomyces pombe, autophagy of ER and mitochondria is induced by nitrogen starvation and is promoted by three Atg20-and Atg24-family proteins - Atg20, Atg24 and SPBC1711.11 (named here as Atg24b). These proteins localize at the pre-autophagosomal structure, or phagophore assembly site (PAS), during starvation. S. pombe Atg24 forms a homo-oligomer and acts redundantly with Atg20 and Atg24b, and the latter two proteins can form a hetero-oligomer. The organelle autophagy defect caused by the loss of these proteins is associated with a reduction of autophagosome size and a decrease in Atg8 accumulation at the PAS. These results provide new insights into the autophagic function of Atg20-and Atg24-family proteins.; National Basic Research Program of China (Ministry of Science and Technology of the People's Republic of China; 973 Program) [2014CB849901, 2014CB849801]; Ministry of Science and Technology of the People's Republic of China; Beijing municipal government; SCI(E); PubMed; ARTICLE; dulilin@nibs.ac.cn; 22; 4289-4304; 129
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/457179]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhao, Dan,Liu, Xiao-Man,Yu, Zhong-Qiu,et al. Atg20-and Atg24-family proteins promote organelle autophagy in fission yeast[J]. JOURNAL OF CELL SCIENCE,2016.
APA	Zhao, Dan.,Liu, Xiao-Man.,Yu, Zhong-Qiu.,Sun, Ling-Ling.,Xiong, Xingchuang.,...&Du, Li-Lin.(2016).Atg20-and Atg24-family proteins promote organelle autophagy in fission yeast.JOURNAL OF CELL SCIENCE.
MLA	Zhao, Dan,et al."Atg20-and Atg24-family proteins promote organelle autophagy in fission yeast".JOURNAL OF CELL SCIENCE (2016).