The ratio of intracellular CRY proteins determines the clock period   length

doi:10.1016/j.bbrc.2016.03.010

CORC > 北京大学 > 生命科学学院

	The ratio of intracellular CRY proteins determines the clock period length
	Li, Yang ; Xiong, Wei ; Zhang, Eric Erquan
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2016
关键词	Circadian rhythms Period Cryptochrome C-terminal tail Protein stability Nuclear importing rate CIRCADIAN CLOCK PHOSPHORYLATION FEEDBACK OSCILLATOR IDENTIFICATION TRANSCRIPTION ROBUSTNESS MUTATION ROLES CELLS
DOI	10.1016/j.bbrc.2016.03.010
英文摘要	Although a deficiency in CRY1 or CRY2 correlates with a shorter or longer circadian period, the regulation of CRY proteins in the circadian period has not been well studied. In this study, we found that both CRY1 and CRY2 were able to rescue oscillation in CRY null cells and that they displayed different periods. Furthermore, we demonstrated that protein nuclear import rates, not protein stability, regulate the period-length at the cellular level. Co-transfection of CRY1 and CRY2 in various ratios in the same cells gives rise to the predicted period length in a dose-dependent manner. Given the distinct characteristics of the C-terminal tails of the CRY1 and CRY2 proteins, our study addresses a long-standing hypothesis that the ratio of these two CRY molecules affects the clock period. (C) 2016 Elsevier Inc. All rights reserved.; 973 Program [2012CB837700]; Beijing Municipal Government; Chinese "Recruitment Program of Global Youth Experts"; SCI(E); PubMed; ARTICLE; zhangerquan@nibs.ac.cn; 3; 531-538; 472
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/434544]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, Yang,Xiong, Wei,Zhang, Eric Erquan. The ratio of intracellular CRY proteins determines the clock period length[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2016.
APA	Li, Yang,Xiong, Wei,&Zhang, Eric Erquan.(2016).The ratio of intracellular CRY proteins determines the clock period length.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.
MLA	Li, Yang,et al."The ratio of intracellular CRY proteins determines the clock period length".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2016).