Identification of EFHD1 as a novel Ca2+ sensor for mitoflash activation

doi:10.1016/j.ceca.2016.03.002

CORC > 北京大学 > 生命科学学院

	Identification of EFHD1 as a novel Ca2+ sensor for mitoflash activation
	Hou, Tingting ; Jian, Chongshu ; Xu, Jiejia ; Huang, August Yue ; Xi, Jianzhong ; Hu, Keping ; Wei, Liping ; Cheng, Heping ; Wang, Xianhua
刊名	CELL CALCIUM
	2016
关键词	Mitochondria Mitochondrial flash (mitoflash) Ca2+-Binding proteins Mitochondrial Ca2+ signaling EFHD1 CA-2& MITOCHONDRIAL CALCIUM UNIPORTER WOLF-HIRSCHHORN-SYNDROME SUPEROXIDE FLASHES PERMEABILITY TRANSITION INDIVIDUAL MITOCHONDRIA SKELETAL-MUSCLE CANDIDATE GENE EF-HAND PROTEIN -INDUCED MEMBRANE TRANSITION
DOI	10.1016/j.ceca.2016.03.002
英文摘要	Mitochondrial flashes (mitoflashes) represent stochastic and discrete mitochondrial events that each comprises a burst of superoxide production accompanied by transient depolarization and matrix alkalinization in a respiratory mitochondrion. While mitochondrial Ca2+ is shown to be an important regulator of mitoflash activity, little is known about its specific mechanism of action. Here we sought to determine possible molecular players that mediate the Ca2+ regulation of mitoflashes by screening mitochondrial proteins containing the Ca2+-binding motifs. In silico analysis and targeted siRNA screening identified four mitoflash, activators (MICUI, EFHDI, SLC25A23, SLC25A25) and one mitoflash inhibitor (LETM1) in terms of their ability to modulate mitoflash response to hyperosmotic stress. In particular, overexpression or down-regulation of EFHD1 enhanced or depressed mitoflash activation, respectively, under various conditions of mitochondrial Ca2+ elevations. Yet, it did not alter mitochondrial Ca2+ handling, mitochondrial respiration, or ROS-induced mitoflash production. Further, disruption of the two EF-hand motifs of EFHD1 abolished its potentiating effect on the mitoflash responses. These results indicate that EFHD1 functions as a novel mitochondrial Ca2+ sensor underlying Ca2+-dependent activation of mitoflashes. (C) 2016 Elsevier Ltd. All rights reserved.; National Key Basic Research Program of China [2013CB531200]; National Science Foundation of China [31130067, 31470811, 31221002, 31327901]; SCI(E); PubMed; ARTICLE; xianhua@pku.edu.cn; 5; 262-270; 59
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/434506]
专题	生命科学学院工学院
推荐引用方式 GB/T 7714	Hou, Tingting,Jian, Chongshu,Xu, Jiejia,et al. Identification of EFHD1 as a novel Ca2+ sensor for mitoflash activation[J]. CELL CALCIUM,2016.
APA	Hou, Tingting.,Jian, Chongshu.,Xu, Jiejia.,Huang, August Yue.,Xi, Jianzhong.,...&Wang, Xianhua.(2016).Identification of EFHD1 as a novel Ca2+ sensor for mitoflash activation.CELL CALCIUM.
MLA	Hou, Tingting,et al."Identification of EFHD1 as a novel Ca2+ sensor for mitoflash activation".CELL CALCIUM (2016).