Bacterial Flagellar Motor Switch in Response to CheY-P Regulation and   Motor Structural Alterations

doi:10.1016/j.bpj.2016.02.023

CORC > 北京大学 > 生命科学学院

	Bacterial Flagellar Motor Switch in Response to CheY-P Regulation and Motor Structural Alterations
	Ma, Qi ; Sowa, Yoshiyuki ; Baker, Matthew A. B. ; Bai, Fan
刊名	BIOPHYSICAL JOURNAL
	2016
关键词	TORQUE-SPEED RELATIONSHIP ESCHERICHIA-COLI CONFORMATIONAL SPREAD ROTARY MOTOR CHEMOTAXIS PROTEINS BINDING MECHANISM FLIG RING
DOI	10.1016/j.bpj.2016.02.023
英文摘要	The bacterial flagellar motor (BFM) is a molecular machine that rotates the helical filaments and propels the bacteria swimming toward favorable conditions. In our previous works, we built a stochastic conformational spread model to explain the dynamic and cooperative behavior of BFM switching. Here, we extended this model to test whether it can explain the latest experimental observations regarding CheY-P regulation and motor structural adaptivity. We show that our model predicts a strong correlation between rotational direction and the number of CheY-Ps bound to the switch complex, in agreement with the latest finding from Fukuoka et al. It also predicts that the switching sensitivity of the BFM can be fine-tuned by incorporating additional units into the switch complex, as recently demonstrated by Yuan et al., who showed that stoichiometry of FliM undergoes dynamic change to maintain ultrasensitivity in the motor switching response. In addition, by locking some rotor switching units on the switch complex into the stable clockwise-only conformation, our model has accurately simulated recent experiments expressing clockwise-locked FliG(Delta PAA) into the switch complex and reproduced the increased switching rate of the motor.; Peking University Project [985]; National Natural Science Foundation of China [31370847]; Fan Bai's inclusion in the Recruitment Program of Global Youth Experts; SCI(E); PubMed; ARTICLE; m.baker@victorchang.edu.au; fbai@pku.edu.cn; 6; 1411-1420; 110
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/434318]
专题	生命科学学院
推荐引用方式 GB/T 7714	Ma, Qi,Sowa, Yoshiyuki,Baker, Matthew A. B.,et al. Bacterial Flagellar Motor Switch in Response to CheY-P Regulation and Motor Structural Alterations[J]. BIOPHYSICAL JOURNAL,2016.
APA	Ma, Qi,Sowa, Yoshiyuki,Baker, Matthew A. B.,&Bai, Fan.(2016).Bacterial Flagellar Motor Switch in Response to CheY-P Regulation and Motor Structural Alterations.BIOPHYSICAL JOURNAL.
MLA	Ma, Qi,et al."Bacterial Flagellar Motor Switch in Response to CheY-P Regulation and Motor Structural Alterations".BIOPHYSICAL JOURNAL (2016).