Methods for PTEN in Stem Cells and Cancer Stem Cells

doi:10.1007/978-1-4939-3299-3_15

CORC > 北京大学 > 生命科学学院

	Methods for PTEN in Stem Cells and Cancer Stem Cells
	Schubbert Suzanne ; Jiao Jing ; Ruscetti Marcus ; Nakashima Jonathan ; Wu Shumin ; Lei Hong ; Xu Qinzhi ; Yi Wenkai ; Zhu Haichuan ; Wu Hong
刊名	Methods in molecular biology (Clifton, N.J.)
	2016
关键词	Cancer stem cells,ES cell,HSC,Leukemia-initiating cell,NSC,PTEN,Prostate cancer stem cell,Stem cell
DOI	10.1007/978-1-4939-3299-3_15
英文摘要	PTEN (phosphatase and tensin homologue) is the first tumor suppressor identified to have phosphatase activity and its gene is the second most frequently deleted or mutated tumor-suppressor gene associated with human cancers. Germline PTEN mutations are the cause of three inherited autosomal dominant disorders. Phosphatidylinositol 3,4,5,-triphosphate (PIP3), the product of the PI3 kinase, is one of the key intracellular targets of PTEN's phosphatase activity, although PTEN's phosphatase-independent activities have also been identified. PTEN is critical for stem cell maintenance, which contributes to its controlled tumorigenesis. PTEN loss leads the development of cancer stem cells (CSCs) that share properties with somatic stem cells, including the capacity for self-renewal and multi-lineage differentiation. Methods to isolate and functionally test stem cells and CSCs are important for understanding PTEN functions and the development of therapeutic approaches to target CSCs without having adverse effects on normal stem cells. Here, we describe protocols for the isolation and functional analysis of PTEN deficient embryonic stem cells, hematopoietic stem cells and leukemia-initiating cells (LICs), neural stem cells, and prostate stem cells and CSCs.; PubMed; 233-85; 1388
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/434299]
专题	生命科学学院
推荐引用方式 GB/T 7714	Schubbert Suzanne,Jiao Jing,Ruscetti Marcus,et al. Methods for PTEN in Stem Cells and Cancer Stem Cells[J]. Methods in molecular biology (Clifton, N.J.),2016.
APA	Schubbert Suzanne.,Jiao Jing.,Ruscetti Marcus.,Nakashima Jonathan.,Wu Shumin.,...&Wu Hong.(2016).Methods for PTEN in Stem Cells and Cancer Stem Cells.Methods in molecular biology (Clifton, N.J.).
MLA	Schubbert Suzanne,et al."Methods for PTEN in Stem Cells and Cancer Stem Cells".Methods in molecular biology (Clifton, N.J.) (2016).