Intrathecal CGRP(8-37)-induced bilateral increase in hindpaw withdrawal   latency in rats with unilateral inflammation

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	Intrathecal CGRP(8-37)-induced bilateral increase in hindpaw withdrawal latency in rats with unilateral inflammation
	Yu, LC ; Hansson, P ; BroddaJansen, G ; Theodorsson, E ; Lundeberg, T
刊名	british journal of pharmacology
	1996
关键词	calcitonin gene-related peptide (CGRP) CGRP(8-37) naloxone carrageenan hindpaw withdrawal latency (HWL) hindpaw withdrawal threshold (HWT) CGRP-like immunoreactivity unilateral experimental inflammation bilateral effects nociceptive test GENE-RELATED PEPTIDE SPINAL DORSAL HORN CARRAGEENAN-INDUCED HYPERALGESIA SUBSTANCE-P NOXIOUS STIMULI SENSORY NEURONS NERVOUS-SYSTEM OPIOID CONTROL CORD RELEASE
英文摘要	1 Recent work in our laboratory has demonstrated that intrathecal administration of a selective antagonist of calcitonin gene-related peptide (CGRP), CGRP(8-37), increased the hindpaw withdrawal latency (HWL) to thermal stimulation and hindpaw withdrawal threshold (HWT) to pressure in normal rats, and that these effects were more pronounced than in rats with mononeuropathy. 2 The present study was performed to investigate the effects of intrathecal administration of CGRP(8-37) on the HWL and HWT in rats with unilateral hindpaw inflammation induced by subcutaneous injection of carrageenin. The effect of naloxone was also studied. 3 Subcutaneous injection of 0.1 ml of carrageenin into the plantar region of the left hindpaw induced a significant increase in the volume of the ipsilateral hindpaw (P< 0.001), and significant bilateral decreases of the HWL to thermal stimulation (ipsilateral: P<0.001; contralateral: P<0.01) and HWT to pressure (ipsilateral: P<0.001; contralateral: P<0.01). 4 Intrathecal administration of 10 nmol of CGRP(8-37), but not of 1 or 5 nmol, induced a significant bilateral increase in the HWL and HWT in rats with experimentally induced inflammation (thermal test: P<0.001; mechanical test: P<0.001). 5 The effect of intrathecal administration of 10 nmol CGRPB(8-37) on HWL and HWT was significantly more pronounced in intact rats than in rats with experimentally induced inflammation (ipsilateral: P<0.001; contralateral: P<0.001). 6 The effect of CGRP(8-37) on withdrawal responses in the inflamed paw was partly reversed by intrathecal injection of naloxone at a dose of 88 nmol in the thermal (ipsilateral: P<0.01; contralateral: P=0.14) and mechanical tests (ipsilateral: P<0.05; contralateral: P=0.60). 7 A significant bilateral increase in the concentration of CGRP-like immunoreactivity in the perfusate of both hindpaws was demonstrated 24 h after unilateral injection of carrageenin (ipsilateral: P<0.001; contralateral: P<0.05). There was also an increase in the amount of CGRP-like immunoreactivity in the cerebrospinal fluid (P<0.001), but not in plasma (P=0.75). 8 The present study demonstrates that acute experimentally-induced unilateral hindpaw inflammation, induces bilateral increases in the amount of CGRP-like immunoreactivity in hindpaw perfusates. Intrathecal administration of CGRP(8-37) increased the HWL to thermal stimulation and HWT to pressure bilaterally. 9 The results indicate that CGRP plays a role in the transmission of presumed nociceptive information in the spinal cord of rats with experimentally induced inflammation. Furthermore, our findings suggest that opioids can modulate CGRP-related effects in the spinal cord.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1996TY67400007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Pharmacology & Pharmacy; SCI(E); 52; ARTICLE; 1; 43-50; 117
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/402806]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yu, LC,Hansson, P,BroddaJansen, G,et al. Intrathecal CGRP(8-37)-induced bilateral increase in hindpaw withdrawal latency in rats with unilateral inflammation[J]. british journal of pharmacology,1996.
APA	Yu, LC,Hansson, P,BroddaJansen, G,Theodorsson, E,&Lundeberg, T.(1996).Intrathecal CGRP(8-37)-induced bilateral increase in hindpaw withdrawal latency in rats with unilateral inflammation.british journal of pharmacology.
MLA	Yu, LC,et al."Intrathecal CGRP(8-37)-induced bilateral increase in hindpaw withdrawal latency in rats with unilateral inflammation".british journal of pharmacology (1996).