Studies on gene structure, enzymatic activity and regulatory mechanism   of acetohydroxy acid isomeroreductase from G2 pea

doi:10.1360/02yc0069

CORC > 北京大学 > 生命科学学院

	Studies on gene structure, enzymatic activity and regulatory mechanism of acetohydroxy acid isomeroreductase from G2 pea
	Xu, YJ ; Gu, XS ; Li, J ; Li, Q ; Davies, PJ ; Zhu, YX
刊名	science in china series c life sciences
	2003
关键词	AAIR G2 pea A/T rich region enzymatic activity senescence ACETOLACTATE SYNTHASE GENE MUSCLE-SPECIFIC ENHANCER G-RICH-BOX EXPRESSION IDENTIFICATION TRANSCRIPTION PURIFICATION INHIBITION PROTEINS CLONING
DOI	10.1360/02yc0069
英文摘要	The AAIR genomic DNA of G2 pea (Pisum sativum L.) was amplified by PCR method. Sequence analysis showed that it was composed of 8 introns and 9 exons with three of the introns containing specific A/T-rich endogenous promoter regions. Molecular hybridization experiments revealed that the expression of AAIR remained at a high level before and after flowering if grown in short day growth chambers. However, when grown under long day conditions, the level of AAIR expression declined very rapidly after flowering. This variation of AAIR expression is consistent with the change of enzymatic activity of acetohydroxy acid isomeroreductase. Functional complementation experiments carried out using an acetohydroxy acid isomeroreductase deficient E. coli strain showed that these cells could not grow on M9 medium without addition of branched-chain amino acids unless they were transformed with the AAIR expression vector. Further study revealed that overexpression of the pea HAIR cDNA in acetohydroxy acid isomeroreductase deficient E. coli strain enhanced significantly its branched-chain amino acid biosynthetic capacity. Results from gel shift experiments showed that fractions of pea nuclear protein extracts could bind specifically to some A/T rich regions present in introns of the HAIR gene. The A/T-rich-region-binding proteins remained at a steady level in the non-senescing apical buds of short-day grown G2 pea. In the rapid-senescing apical buds of long-day grown G2 pea, the levels of these proteins declined rapidly after flower initiation. Therefore, the nuclear protein binding capacities to endogenous promoter regions may constitute an important mechanism to regulate AAIR gene expression.; Biology; SCI(E); EI; 0; 4; 389-398; 46
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/401043]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xu, YJ,Gu, XS,Li, J,et al. Studies on gene structure, enzymatic activity and regulatory mechanism of acetohydroxy acid isomeroreductase from G2 pea[J]. science in china series c life sciences,2003.
APA	Xu, YJ,Gu, XS,Li, J,Li, Q,Davies, PJ,&Zhu, YX.(2003).Studies on gene structure, enzymatic activity and regulatory mechanism of acetohydroxy acid isomeroreductase from G2 pea.science in china series c life sciences.
MLA	Xu, YJ,et al."Studies on gene structure, enzymatic activity and regulatory mechanism of acetohydroxy acid isomeroreductase from G2 pea".science in china series c life sciences (2003).