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	Transformation of Dendrobium phalaenopsis Fitz. and D-nobile Lindl. with CHS and AP1 genes
	Nie, XR ; Men, SZ ; An, LX ; Li, M ; Wei, CH ; Chen, QB ; Li, Y
	2005
关键词	Agrobacterium tumefaciens AP1 gene CHS gene flower color flowering time protocorm-like bodies transgenic INHIBITS FLOWER PIGMENTATION CHALCONE SYNTHASE GENE PARTICLE BOMBARDMENT MEDIATED TRANSFORMATION ORCHID AGROBACTERIUM ARABIDOPSIS PLANTS PROTOCORMS EVOLUTION
英文摘要	To alter the flower color and flowering time of ornamental orchids Dendrobium phalaerlopsis and D. nobile, we transformed the orchids of Dendrobium phalaenopsis and D. nobile with the CHSA gene and the floral meristem identity gene AP1 via Agrobacterium-mediated transformation of protocorm-like bodies (PLBs), respectively. The CHSA gene was cloned from Petunia hybrid, and the AP1 gene was cloned from Arabidopsis. A total of 69 putative transformed lines of D. nobile with the AP1 gene were obtained, and 7 lines had developed roots. While for the CHSA gene, four independent transgenic lines of Dendrobium phalaenopsis were obtained, and three lines had developed roots. PCR analysis detected the CHSA fragment in all the 4 putative transgenic lines, while in untransformed control plants no specific products were detected. Similarly Southern blot analysis of transformants with the AP1 gene showed that putative transgenic lines have hybridization signals, while no hybridization signal for AP1 gene was detected in untransformed control plants.; Horticulture; SCI(E); 2; ARTICLE; 2; 89-94; 5
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/399365]
专题	生命科学学院
推荐引用方式 GB/T 7714	Nie, XR,Men, SZ,An, LX,et al. Transformation of Dendrobium phalaenopsis Fitz. and D-nobile Lindl. with CHS and AP1 genes[J],2005.
APA	Nie, XR.,Men, SZ.,An, LX.,Li, M.,Wei, CH.,...&Li, Y.(2005).Transformation of Dendrobium phalaenopsis Fitz. and D-nobile Lindl. with CHS and AP1 genes..
MLA	Nie, XR,et al."Transformation of Dendrobium phalaenopsis Fitz. and D-nobile Lindl. with CHS and AP1 genes".(2005).

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