Suppression of 9-cis-Epoxycarotenoid Dioxygenase, Which Encodes a Key   Enzyme in Abscisic Acid Biosynthesis, Alters Fruit Texture in Transgenic   Tomato

doi:10.1104/pp.111.186866

CORC > 北京大学 > 生命科学学院

	Suppression of 9-cis-Epoxycarotenoid Dioxygenase, Which Encodes a Key Enzyme in Abscisic Acid Biosynthesis, Alters Fruit Texture in Transgenic Tomato
	Sun, Liang ; Sun, Yufei ; Zhang, Mei ; Wang, Ling ; Ren, Jie ; Cui, Mengmeng ; Wang, Yanping ; Ji, Kai ; Li, Ping ; Li, Qian ; Chen, Pei ; Dai, Shengjie ; Duan, Chaorui ; Wu, Yan ; Leng, Ping
刊名	plant physiology
	2012
关键词	PLANT-CELL WALLS XYLOGLUCAN ENDO-TRANSGLYCOSYLASE PECTIN METHYLESTERASE GENE GRAPE BERRY DEVELOPMENT CHELATASE H-SUBUNIT BETA-GALACTOSIDASE DROUGHT STRESS ANTISENSE RNA POLYURONIDE DEGRADATION POLYGALACTURONASE GENE
DOI	10.1104/pp.111.186866
英文摘要	Cell wall catabolism during fruit ripening is under complex control and is key for fruit quality and shelf life. To examine the role of abscisic acid (ABA) in tomato (Solanum lycopersicum) fruit ripening, we suppressed SlNCED1, which encodes 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme in the biosynthesis of ABA. To suppress SlNCED1 specifically in tomato fruits, and thus avoid the pleiotropic phenotypes associated with ABA deficiency, we used an RNA interference construct driven by the fruit-specific E8 promoter. ABA accumulation and SlNCED1 transcript levels in the transgenic fruit were down-regulated to between 20% and 50% of the levels measured in the control fruit. This significant reduction in NCED activity led to a down-regulation in the transcription of genes encoding major cell wall catabolic enzymes, specifically polygalacturonase (SlPG), pectin methyl esterase (SlPME), beta-galactosidase precursor mRNA (SlTBG), xyloglucan endotransglycosylase (SlXET), endo-1,4-beta-cellulose (SlCels), and expansin (SlExp). This resulted in an increased accumulation of pectin during ripening. In turn, this led to a significant extension of the shelf life to 15 to 29 d compared with a shelf life of only 7 d for the control fruit and an enhancement of fruit firmness at the mature stage by 30% to 45%. In conclusion, ABA affects cell wall catabolism during tomato fruit ripening via down-regulation of the expression of major catabolic genes (SlPG, SlPME, SlTBG, SlXET, SlCels, and SlExp).; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000298662500025&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Plant Sciences; SCI(E); 70; ARTICLE; 1; 283-298; 158
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/393999]
专题	生命科学学院
推荐引用方式 GB/T 7714	Sun, Liang,Sun, Yufei,Zhang, Mei,et al. Suppression of 9-cis-Epoxycarotenoid Dioxygenase, Which Encodes a Key Enzyme in Abscisic Acid Biosynthesis, Alters Fruit Texture in Transgenic Tomato[J]. plant physiology,2012.
APA	Sun, Liang.,Sun, Yufei.,Zhang, Mei.,Wang, Ling.,Ren, Jie.,...&Leng, Ping.(2012).Suppression of 9-cis-Epoxycarotenoid Dioxygenase, Which Encodes a Key Enzyme in Abscisic Acid Biosynthesis, Alters Fruit Texture in Transgenic Tomato.plant physiology.
MLA	Sun, Liang,et al."Suppression of 9-cis-Epoxycarotenoid Dioxygenase, Which Encodes a Key Enzyme in Abscisic Acid Biosynthesis, Alters Fruit Texture in Transgenic Tomato".plant physiology (2012).