Structure of the mRNA splicing complex component Cwc2: insights into RNA   recognition

doi:10.1042/BJ20111385

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	Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition
	Lu, Peilong ; Lu, Guifeng ; Yan, Chuangye ; Wang, Li ; Li, Wenqi ; Yin, Ping
刊名	biochemical journal
	2012
关键词	RNA recognition motif nineteen complex (NTC) spliceosome structural biology zinc finger ZINC-FINGER PROTEINS AU-RICH ELEMENT FUNCTIONAL-ANALYSIS U6 SNRNA HNRNP-F SPLICEOSOME MOTIFS SOFTWARE DOMAIN INTERACTS
DOI	10.1042/BJ20111385
英文摘要	The Prp19-associated complex [NTC (nineteen complex)] plays a crucial role in intron removal during premature mRNA splicing in eukaryotes. Only one component of the NTC, Cwc2, is capable of binding RNA. In the present study we report the 1.9 angstrom (1 angstrom = 0.1 nm) X-ray structure of the Cwc2 core domain, which is both necessary and sufficient for RNA binding. The Cwc2 core domain contains two sub-domains, a CCCH-type ZnF (zinc finger) and a RRM (RNA recognition motif). Unexpectedly, the ZnF domain and the RRM form a single folding unit, glued together by extensive hydrophobic interactions and hydrogen bonds. Structure-guided mutational analysis revealed that the intervening loop [known as the RB loop (RNA-binding loop)] between ZnF and RRM plays an essential role in RNA binding. In addition, a number of highly conserved positively charged residues on the beta-strands of RRM make an important contribution to RNA binding. Intriguingly, these residues and a portion of the RB loop constitute an extended basic surface strip that encircles Cwc2 halfway. The present study serves as a framework for understanding the regulatory function of the NTC in RNA splicing.; Biochemistry & Molecular Biology; SCI(E); 6; ARTICLE; 591-597; 441
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/393769]
专题	生命科学学院
推荐引用方式 GB/T 7714	Lu, Peilong,Lu, Guifeng,Yan, Chuangye,et al. Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition[J]. biochemical journal,2012.
APA	Lu, Peilong,Lu, Guifeng,Yan, Chuangye,Wang, Li,Li, Wenqi,&Yin, Ping.(2012).Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition.biochemical journal.
MLA	Lu, Peilong,et al."Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition".biochemical journal (2012).