Crystal structure of ISG54 reveals a novel RNA binding structure and   potential functional mechanisms

doi:10.1038/cr.2012.111

CORC > 北京大学 > 生命科学学院

	Crystal structure of ISG54 reveals a novel RNA binding structure and potential functional mechanisms
	Yang, Zhenlin ; Liang, Huanhuan ; Zhou, Qian ; Li, Ying ; Chen, Haiwei ; Ye, Wen ; Chen, Danying ; Fleming, Joy ; Shu, Hongbing ; Liu, Yingfang
刊名	细胞研究英文版
	2012
关键词	structure biology ISG54 INTERFERON-STIMULATED GENES AU-RICH ELEMENTS MESSENGER-RNA MOLECULAR-CLONING INDUCED PROTEIN TPR VIRUS SEQUENCE REPEAT FAMILY
DOI	10.1038/cr.2012.111
英文摘要	Interferon-stimulated gene 56 (ISG56) family members play important roles in blocking viral replication and regulating cellular functions, however, their underlying molecular mechanisms are largely unclear. Here, we present the crystal structure of ISG54, an ISG56 family protein with a novel RNA-binding structure. The structure shows that ISG54 monomers have 9 tetratricopeptide repeat-like motifs and associate to form domain-swapped dimers. The C-terminal part folds into a super-helical structure and has an extensively positively-charged nucleotide-binding channel on its inner surface. EMSA results show that ISG54 binds specifically to some RNAs, such as adenylate uridylate (AU)-rich RNAs, with or without 5' triphosphorylation. Mutagenesis and functional studies show that this RNA-binding ability is important to its antiviral activity. Our results suggest a new mechanism underlying the antiviral activity of this interferon-inducible gene 56 family member.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000308611900006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Cell Biology; SCI(E); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 27; ARTICLE; 9; 1328-1338; 22
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/392953]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yang, Zhenlin,Liang, Huanhuan,Zhou, Qian,et al. Crystal structure of ISG54 reveals a novel RNA binding structure and potential functional mechanisms[J]. 细胞研究英文版,2012.
APA	Yang, Zhenlin.,Liang, Huanhuan.,Zhou, Qian.,Li, Ying.,Chen, Haiwei.,...&Liu, Yingfang.(2012).Crystal structure of ISG54 reveals a novel RNA binding structure and potential functional mechanisms.细胞研究英文版.
MLA	Yang, Zhenlin,et al."Crystal structure of ISG54 reveals a novel RNA binding structure and potential functional mechanisms".细胞研究英文版 (2012).