IBM1, a JmjC domain-containing histone demethylase, is involved in the   regulation of RNA-directed DNA methylation through the epigenetic   control of RDR2 and DCL3 expression in Arabidopsis

doi:10.1093/nar/gks647

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	IBM1, a JmjC domain-containing histone demethylase, is involved in the regulation of RNA-directed DNA methylation through the epigenetic control of RDR2 and DCL3 expression in Arabidopsis
	Fan, Di ; Dai, Yan ; Wang, Xuncheng ; Wang, Zhenjie ; He, Hang ; Yang, Hongchun ; Cao, Ying ; Deng, Xing Wang ; Ma, Ligeng
刊名	核酸研究
	2012
关键词	LOCUS-SPECIFIC CONTROL CONTAINING PROTEINS PROCESSING CENTER INVERTED REPEAT HUMAN GENOME THALIANA GENES PLANTS SIRNA METHYLTRANSFERASE
DOI	10.1093/nar/gks647
英文摘要	Small RNA-directed DNA methylation (RdDM) is an important epigenetic pathway in Arabidopsis that controls the expression of multiple genes and several developmental processes. RNA-DEPENDENT RNA POLYMERASE 2 (RDR2) and DICER-LIKE 3 (DCL3) are necessary factors in 24-nt small interfering RNA (siRNA) biogenesis, which is part of the RdDM pathway. Here, we found that Increase in BONSAI Methylation 1 (IBM1), a conserved JmjC family histone demethylase, is directly associated with RDR2 and DCL3 chromatin. The mutation of IBM1 induced the hypermethylation of H3K9 and DNA non-CG sites within RDR2 and DCL3, which repressed their expression. A genome-wide analysis suggested that the reduction in RDR2 and DCL3 expression affected siRNA biogenesis in a locus-specific manner and disrupted RdDM-directed gene repression. Together, our results suggest that IBM1 regulates gene expression through two distinct pathways: direct association to protect genes from silencing by preventing the coupling of histone and DNA methylation, and indirect silencing of gene expression through RdDM-directed repression.; Biochemistry & Molecular Biology; SCI(E); 6; ARTICLE; 18; 8905-8916; 40
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/392829]
专题	生命科学学院
推荐引用方式 GB/T 7714	Fan, Di,Dai, Yan,Wang, Xuncheng,et al. IBM1, a JmjC domain-containing histone demethylase, is involved in the regulation of RNA-directed DNA methylation through the epigenetic control of RDR2 and DCL3 expression in Arabidopsis[J]. 核酸研究,2012.
APA	Fan, Di.,Dai, Yan.,Wang, Xuncheng.,Wang, Zhenjie.,He, Hang.,...&Ma, Ligeng.(2012).IBM1, a JmjC domain-containing histone demethylase, is involved in the regulation of RNA-directed DNA methylation through the epigenetic control of RDR2 and DCL3 expression in Arabidopsis.核酸研究.
MLA	Fan, Di,et al."IBM1, a JmjC domain-containing histone demethylase, is involved in the regulation of RNA-directed DNA methylation through the epigenetic control of RDR2 and DCL3 expression in Arabidopsis".核酸研究 (2012).