Probing Meiotic Recombination and Aneuploidy of Single Sperm Cells by   Whole-Genome Sequencing

doi:10.1126/science.1229112

CORC > 北京大学 > 生命科学学院

	Probing Meiotic Recombination and Aneuploidy of Single Sperm Cells by Whole-Genome Sequencing
	Lu, Sijia ; Zong, Chenghang ; Fan, Wei ; Yang, Mingyu ; Li, Jinsen ; Chapman, Alec R. ; Zhu, Ping ; Hu, Xuesong ; Xu, Liya ; Yan, Liying ; Bai, Fan ; Qiao, Jie ; Tang, Fuchou ; Li, Ruiqiang ; Xie, X. Sunney
刊名	science
	2012
关键词	IN-SITU HYBRIDIZATION HOT-SPOTS CHROMOSOME-Y HUMANS MEIOSIS RATES MAP AMPLIFICATION CHIMPANZEES PATTERNS
DOI	10.1126/science.1229112
英文摘要	Meiotic recombination creates genetic diversity and ensures segregation of homologous chromosomes. Previous population analyses yielded results averaged among individuals and affected by evolutionary pressures. We sequenced 99 sperm from an Asian male by using the newly developed amplification method-multiple annealing and looping-based amplification cycles-to phase the personal genome and map recombination events at high resolution, which are nonuniformly distributed across the genome in the absence of selection pressure. The paucity of recombination near transcription start sites observed in individual sperm indicates that such a phenomenon is intrinsic to the molecular mechanism of meiosis. Interestingly, a decreased crossover frequency combined with an increase of autosomal aneuploidy is observable on a global per-sperm basis.; Multidisciplinary Sciences; SCI(E); 54; ARTICLE; 6114; 1627-1630; 338
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/392440]
专题	生命科学学院
推荐引用方式 GB/T 7714	Lu, Sijia,Zong, Chenghang,Fan, Wei,et al. Probing Meiotic Recombination and Aneuploidy of Single Sperm Cells by Whole-Genome Sequencing[J]. science,2012.
APA	Lu, Sijia.,Zong, Chenghang.,Fan, Wei.,Yang, Mingyu.,Li, Jinsen.,...&Xie, X. Sunney.(2012).Probing Meiotic Recombination and Aneuploidy of Single Sperm Cells by Whole-Genome Sequencing.science.
MLA	Lu, Sijia,et al."Probing Meiotic Recombination and Aneuploidy of Single Sperm Cells by Whole-Genome Sequencing".science (2012).