诱导风信子再生花芽不断分化花被片的研究

CORC > 北京大学 > 生命科学学院

	诱导风信子再生花芽不断分化花被片的研究; Induction of continuous tepal differentiation from in vitro regenerated flower buds of Hyacinthus orientalis
	陆文樑 ; 白书农 ; 张宪省
刊名	植物学报
	1999
关键词	风信子离体培养花芽直接再生花被片持续分化 Hyacinthus orientalis culture in vitro direct regeneration of flower buds continuous differentiation of tepals
英文摘要	通过外源激素及外植体年龄的控制，诱导风信子（ＨｙａｃｉｎｔｈｕｓｏｒｉｅｎｔａｌｉｓＬ．）再生花芽不断分化花被片已经获得成功。在２５０ｄ的继代培养中平均每个花芽可分化７０多片花被片，最多的可分化１４０多片。对这种花芽不断分化花被片的形态发生过程以及生长发育特点的观察表明，花芽的第１轮器官与风信子野生型花基本相同，是花被，它由花被筒及其上部的裂片———花被片组成。第２轮和第３轮器官在野生型中应该是雄蕊和雌蕊，但再生花芽仍然分化花被片。这种花芽的生长与发育的速度在开始的几个月中较快，以后随培养天数增加而不断减慢。继代培养１５０ｄ以后，花芽生长与器官分化肉眼已很难鉴别。在...; Continuous differentiation of tepals was successively induced from regenerated flower buds in Hyacinthus orientalis L. cv. White Pearl by controlling the exogenous hormones and explant ages. In 250 days of subculture, each flower bud differentiated an average of more than 70 tepals, with a maximum of over 140 tepals. Studies on the morphogenesis and characteristics of growth and development of the flower buds indicate that the first whorled organ of the flower bud was perianth which consisted of perianth tube and tepals grown at the top of the perianth tube, which is the same as the flower bud of the wild type in H, orentalis. The second and third whorls of the flower bud, which should be stamen and pistil in the wild type, but remained as the tepals in the regenerated flower bud. Growth of the regenerated flower bud was faster in the first several months of culture, then slowed down gradually with time. After 150 days in culture the flower bud growth and organ differentiation became very slow. Other than the tepal differentiation the regenerated flower buds also differentiated at random positions some small flower buds that also differentiated the tepals only. Histological observation revealed that the origin of the regenerated flower buds was jointly participated by some cells in the epidermal and subepidermal layers at the inner surface of the perianth explant, and the inner small flower buds were originated from the meristem which was formed by the transformation of the parenchyma at the base of the very young tepal, The authors also compared and discussed the similarities and differences of the phenotypes between the regenerated flower bud in Hyacinthus and agamous flower in Arabidopsis, from which, they have hypothesized on the role of the hormones in the promotion and termination of the gene expressions by an order of development in plant.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000083887700002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); 中文核心期刊要目总览(PKU); 中国科学引文数据库(CSCD); 13; 09; 921-1028; 41
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/389742]
专题	生命科学学院
推荐引用方式 GB/T 7714	陆文樑,白书农,张宪省. 诱导风信子再生花芽不断分化花被片的研究, Induction of continuous tepal differentiation from in vitro regenerated flower buds of Hyacinthus orientalis[J]. 植物学报,1999.
APA	陆文樑,白书农,&张宪省.(1999).诱导风信子再生花芽不断分化花被片的研究.植物学报.
MLA	陆文樑,et al."诱导风信子再生花芽不断分化花被片的研究".植物学报 (1999).