Assembly of photosystem II. Inactivation of the rubA gene encoding a   membrane-associated rubredoxin in the cyanobacterium Synechococcus sp   PCC 7002 causes a loss of photosystem I activity

doi:10.1074/jbc.M201103200

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	Assembly of photosystem II. Inactivation of the rubA gene encoding a membrane-associated rubredoxin in the cyanobacterium Synechococcus sp PCC 7002 causes a loss of photosystem I activity
	Shen, GZ ; Zhao, JD ; Reimer, SK ; Antonkine, ML ; Cai, Q ; Weiland, SM ; Golbeck, JH ; Bryant, DA
刊名	journal of biological chemistry
	2002
关键词	OXIDATIVE STRESS PROTECTION AMINO-ACID-SEQUENCE OPEN READING FRAMES DESULFOVIBRIO-VULGARIS ARABIDOPSIS-THALIANA NUCLEOTIDE-SEQUENCE ANGSTROM RESOLUTION ELECTRON-TRANSFER ESCHERICHIA-COLI CRYPTOMONAD ALGA
DOI	10.1074/jbc.M201103200
英文摘要	A 4.4-kb HindIII fragment, encoding an unusual rubredoxin (denoted RubA), a homolog of the Synechocystis sp. PCC 6803 gene slr2034 and Arabidopsis thaliana HCF136, and the psbEFLJ operon, was cloned from the cyanobacterium Synechococcus sp. PCC 7002. Inactivation of the slr2034 homolog produced a mutant with no detectable phenotype and wild-type photosystem (PS) II levels. Inactivation of the rubA gene of Synechococcus sp. PCC 7002 produced a mutant unable to grow photoautotrophically. RubA and PS I electron transport activity were completely absent in the mutant, although PS 11 activity was similar to80% of the wild-type level. RubA contains a domain of similar to50 amino acids with very high similarity to the rubredoxins of anaerobic bacteria and archaea, but it also contains a region of about 50 amino acids that is predicted to form a flexible hinge and a transmembrane a-helix at its C terminus. Overproduction of the water-soluble rubredoxin domain in Escherichia coli led to a product with the absorption and EPR spectra of typical rubredoxins. RubA was present in thylakoid but not plasma membranes of cyanobacteria and in chloroplast thylakoids isolated from spinach and Chlamydomonas reinhardtii. Fractionation studies suggest that RubA might transiently associate with PS I monomers, but no evidence for an association with PS I trimers or PS II was observed. PS I levels were significantly lower than in the wild type (similar to40%), but trimeric PS I complexes could be isolated from the rubA mutant. These PS I complexes completely lacked the stromal subunits PsaC, PsaD), and PsaE but contained all membrane-intrinsic subunits. The three missing proteins could be detected immunologically in whole cells, but their levels were greatly reduced, and degradation products were also detected. Our results indicate that RubA plays a specific role in the biogenesis of PS I.; Biochemistry & Molecular Biology; SCI(E); 50; ARTICLE; 23; 20343-20354; 277
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/388449]
专题	生命科学学院
推荐引用方式 GB/T 7714	Shen, GZ,Zhao, JD,Reimer, SK,et al. Assembly of photosystem II. Inactivation of the rubA gene encoding a membrane-associated rubredoxin in the cyanobacterium Synechococcus sp PCC 7002 causes a loss of photosystem I activity[J]. journal of biological chemistry,2002.
APA	Shen, GZ.,Zhao, JD.,Reimer, SK.,Antonkine, ML.,Cai, Q.,...&Bryant, DA.(2002).Assembly of photosystem II. Inactivation of the rubA gene encoding a membrane-associated rubredoxin in the cyanobacterium Synechococcus sp PCC 7002 causes a loss of photosystem I activity.journal of biological chemistry.
MLA	Shen, GZ,et al."Assembly of photosystem II. Inactivation of the rubA gene encoding a membrane-associated rubredoxin in the cyanobacterium Synechococcus sp PCC 7002 causes a loss of photosystem I activity".journal of biological chemistry (2002).