Expression, purification and characterization of human prorelaxin-like-protein H2 in Escherichia coli

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	Expression, purification and characterization of human prorelaxin-like-protein H2 in Escherichia coli
	Chen Li-Ming ; Yang Xing-Wen ; Tang Jian-Guo
刊名	sheng wu hua xue yu sheng wu wu li xue bao acta biochimica et biophysica sinica
	2002
英文摘要	The cDNA gene encoding human prorelaxin H2 was subcloned from plasmid pMAL p2X-hRLX H2 into the EcoRI and BamHI sites of prokaryotic expression vector pBV220, resulting in the recombinant plasmid pBV220-hPRLX H2, which was subsequently transformed into Escherichia coli DH5arg;. The target gene was highly expressed in the form of inclusion body by thermoinduction at 42 degrees. Recombinant human Met-prorelaxin-like-protein H2 was refolded in vitro, purified by Sephadex G-75 gel filtration chromatography and reverse phase fast protein liquid chromatography. The yield was approximately 2-3 mg/L. The purified recombinant human Met-prorelaxin-like-protein H2 was shown to be a single band by 15% SDS-PAGE and gave correct amino acid composition of Met-prorelaxin. Molecular weight of the purified recombinant human Met-prorelaxin-like-protein H2 was measured to be 18 390.4 (calculated theoretical value 18 392.3) by matrix assisted laser desorption ionization time-of-flight mass spectroscopy.; SCI(E); PubMed; 0; ARTICLE; 5; 595-600; 34
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/347296]
专题	生命科学学院
推荐引用方式 GB/T 7714	Chen Li-Ming,Yang Xing-Wen,Tang Jian-Guo. Expression, purification and characterization of human prorelaxin-like-protein H2 in Escherichia coli[J]. sheng wu hua xue yu sheng wu wu li xue bao acta biochimica et biophysica sinica,2002.
APA	Chen Li-Ming,Yang Xing-Wen,&Tang Jian-Guo.(2002).Expression, purification and characterization of human prorelaxin-like-protein H2 in Escherichia coli.sheng wu hua xue yu sheng wu wu li xue bao acta biochimica et biophysica sinica.
MLA	Chen Li-Ming,et al."Expression, purification and characterization of human prorelaxin-like-protein H2 in Escherichia coli".sheng wu hua xue yu sheng wu wu li xue bao acta biochimica et biophysica sinica (2002).