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	Sequence analysis of keratin-like proteins and cloning of intermediate filament-like cDNA from higher plant cells
	Zhao, DZ ; Chen, DY ; Yang, C ; Zhai, ZH
刊名	science in china series c life sciences
	2000
关键词	Daucus carota L. intermediate filament keratin-like protein protein purification amino acid sequence analysis cDNA cloning 14-NM FILAMENT
DOI	10.1007/BF02879285
英文摘要	Two keratin-like proteins of 64 and 55 ku were purified from suspension cells of Daucus carota L., and their partial amino acid sequences were determined. The homological analysis showed that the sequence from the 64 ku protein was highly homological to beta-glucosidase, and that from the 55 ku protein had no significant homologue in GenBank. Using conservative sequence of animal IF proteins as primer, we cloned a cDNA fragment from Daucus carota L. Southern blot and Northern blot results indicated that this cDNA fragment was a single copy gene and expressed both in suspension cells and leaves. Homological analysis revealed that it had moderate homology to a variety of alpha-helical proteins. Our results might shed more light on molecular characterization of IF existence in higher plant.; Biology; SCI(E); PubMed; 1; ARTICLE; 3; 265-271; 43
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/345111]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhao, DZ,Chen, DY,Yang, C,et al. Sequence analysis of keratin-like proteins and cloning of intermediate filament-like cDNA from higher plant cells[J]. science in china series c life sciences,2000.
APA	Zhao, DZ,Chen, DY,Yang, C,&Zhai, ZH.(2000).Sequence analysis of keratin-like proteins and cloning of intermediate filament-like cDNA from higher plant cells.science in china series c life sciences.
MLA	Zhao, DZ,et al."Sequence analysis of keratin-like proteins and cloning of intermediate filament-like cDNA from higher plant cells".science in china series c life sciences (2000).

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