Comparative Proteomics Indicates That Biosynthesis of Pectic Precursors   Is Important for Cotton Fiber and Arabidopsis Root Hair Elongation

doi:10.1074/mcp.M110.000349

CORC > 北京大学 > 生命科学学院

	Comparative Proteomics Indicates That Biosynthesis of Pectic Precursors Is Important for Cotton Fiber and Arabidopsis Root Hair Elongation
	Pang, Chao-You ; Wang, Hui ; Pang, Yu ; Xu, Chao ; Jiao, Yue ; Qin, Yong-Mei ; Western, Tamara L. ; Yu, Shu-Xun ; Zhu, Yu-Xian
刊名	molecular cellular proteomics
	2010
关键词	CELL-WALL BIOSYNTHESIS GENE-EXPRESSION 4-EPIMERASE ISOFORMS STUDIES REVEAL SEED COAT ETHYLENE GROWTH THALIANA MUTATION IDENTIFICATION
DOI	10.1074/mcp.M110.000349
英文摘要	The quality of cotton fiber is determined by its final length and strength, which is a function of primary and secondary cell wall deposition. Using a comparative proteomics approach, we identified 104 proteins from cotton ovules 10 days postanthesis with 93 preferentially accumulated in the wild type and 11 accumulated in the fuzzless-lintless mutant. Bioinformatics analysis indicated that nucleotide sugar metabolism was the most significantly upregulated biochemical process during fiber elongation. Seven protein spots potentially involved in pectic cell wall polysaccharide biosynthesis were specifically accumulated in wild-type samples at both the protein and transcript levels. Protein and mRNA expression of these genes increased when either ethylene or lignoceric acid (C24:0) was added to the culture medium, suggesting that these compounds may promote fiber elongation by modulating the production of cell wall polymers. Quantitative analysis revealed that fiber primary cell walls contained significantly higher amounts of pectin, whereas more hemicellulose was found in ovule samples. Significant fiber growth was observed when UDP-L-rhamnose, UDP-D-galacturonic acid, or UDP-D-glucuronic acid, all of which were readily incorporated into the pectin fraction of cell wall preparations, was added to the ovule culture medium. The short root hairs of Arabidopsis uer1-1 and gae6-1 mutants were complemented either by genetic transformation of the respective cotton cDNA or by adding a specific pectin precursor to the growth medium. When two pectin precursors, produced by either UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase 4-reductase or by UDP-D-glucose dehydrogenase and UDP-D-glucuronic acid 4-epimerase successively, were used in the chemical complementation assay, wild-type root hair lengths were observed in both cut1 and ein2-5 Arabidopsis seedlings, which showed defects in C24:0 biosynthesis or ethylene signaling, respectively. Our results suggest that ethylene and C24:0 may promote cotton fiber and Arabidopsis root hair growth by activating the pectin biosynthesis network, especially UDP-L-rhamnose and UDP-D-galacturonic acid synthesis. Molecular & Cellular Proteomics 9:2019-2033, 2010.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000281562100017&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; SCI(E); PubMed; 80; ARTICLE; 9; 2019-2033; 9
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/344228]
专题	生命科学学院
推荐引用方式 GB/T 7714	Pang, Chao-You,Wang, Hui,Pang, Yu,et al. Comparative Proteomics Indicates That Biosynthesis of Pectic Precursors Is Important for Cotton Fiber and Arabidopsis Root Hair Elongation[J]. molecular cellular proteomics,2010.
APA	Pang, Chao-You.,Wang, Hui.,Pang, Yu.,Xu, Chao.,Jiao, Yue.,...&Zhu, Yu-Xian.(2010).Comparative Proteomics Indicates That Biosynthesis of Pectic Precursors Is Important for Cotton Fiber and Arabidopsis Root Hair Elongation.molecular cellular proteomics.
MLA	Pang, Chao-You,et al."Comparative Proteomics Indicates That Biosynthesis of Pectic Precursors Is Important for Cotton Fiber and Arabidopsis Root Hair Elongation".molecular cellular proteomics (2010).