Get Phases from Arsenic Anomalous Scattering: de novo SAD Phasing of Two   Protein Structures Crystallized in Cacodylate Buffer

doi:10.1371/journal.pone.0024227

CORC > 北京大学 > 生命科学学院

	Get Phases from Arsenic Anomalous Scattering: de novo SAD Phasing of Two Protein Structures Crystallized in Cacodylate Buffer
	Liu, Xiang ; Zhang, Heng ; Wang, Xiao-Jun ; Li, Lan-Fen ; Su, Xiao-Dong
刊名	plos one
	2011
关键词	HEAVY-ATOM DERIVATIVES ACTIVE-SITE MACROMOLECULAR CRYSTALLOGRAPHY ANGSTROM RESOLUTION JOLLY SAD MECHANISM POWER MAD ACTIVATION P40(PHOX)
DOI	10.1371/journal.pone.0024227
英文摘要	The crystal structures of two proteins, a putative pyrazinamidase/nicotinamidase from the dental pathogen Streptococcus mutans (SmPncA) and the human caspase-6 (Casp6), were solved by de novo arsenic single-wavelength anomalous diffraction (As-SAD) phasing method. Arsenic (As), an uncommonly used element in SAD phasing, was covalently introduced into proteins by cacodylic acid, the buffering agent in the crystallization reservoirs. In SmPncA, the only cysteine was bound to dimethylarsinoyl, which is a pentavalent arsenic group (As (V)). This arsenic atom and a protein-bound zinc atom both generated anomalous signals. The predominant contribution, however, was from the As anomalous signals, which were sufficient to phase the SmPncA structure alone. In Casp6, four cysteines were found to bind cacodyl, a trivalent arsenic group (As (III)), in the presence of the reducing agent, dithiothreitol (DTT), and arsenic atoms were the only anomalous scatterers for SAD phasing. Analyses and discussion of these two As-SAD phasing examples and comparison of As with other traditional heavy atoms that generate anomalous signals, together with a few arsenic-based de novo phasing cases reported previously strongly suggest that As is an ideal anomalous scatterer for SAD phasing in protein crystallography.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000294686100025&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); PubMed; 6; ARTICLE; 9; e24227; 6
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/343768]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu, Xiang,Zhang, Heng,Wang, Xiao-Jun,et al. Get Phases from Arsenic Anomalous Scattering: de novo SAD Phasing of Two Protein Structures Crystallized in Cacodylate Buffer[J]. plos one,2011.
APA	Liu, Xiang,Zhang, Heng,Wang, Xiao-Jun,Li, Lan-Fen,&Su, Xiao-Dong.(2011).Get Phases from Arsenic Anomalous Scattering: de novo SAD Phasing of Two Protein Structures Crystallized in Cacodylate Buffer.plos one.
MLA	Liu, Xiang,et al."Get Phases from Arsenic Anomalous Scattering: de novo SAD Phasing of Two Protein Structures Crystallized in Cacodylate Buffer".plos one (2011).