A Small Heat Shock Protein Enables Escherichia coli To Grow at a Lethal   Temperature of 50 degrees C Conceivably by Maintaining Cell Envelope   Integrity

doi:10.1128/JB.01473-14

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	A Small Heat Shock Protein Enables Escherichia coli To Grow at a Lethal Temperature of 50 degrees C Conceivably by Maintaining Cell Envelope Integrity
	Ezemaduka, Anastasia N. ; Yu, Jiayu ; Shi, Xiaodong ; Zhang, Kaiming ; Yin, Chang-Cheng ; Fu, Xinmiao ; Chang, Zengyi
刊名	journal of bacteriology
	2014
关键词	CHAPERONE-LIKE ACTIVITY MYCOBACTERIUM-TUBERCULOSIS CAENORHABDITIS-ELEGANS ENHANCES THERMOTOLERANCE ELEVATED-TEMPERATURES MOLECULAR CHAPERONES PLASMA-MEMBRANE GENE EVOLUTION HSP16.3
DOI	10.1128/JB.01473-14
英文摘要	It is essential for organisms to adapt to fluctuating growth temperatures. Escherichia coli, a model bacterium commonly used in research and industry, has been reported to grow at a temperature lower than 46.5 degrees C. Here we report that the heterologous expression of the 17-kDa small heat shock protein from the nematode Caenorhabditis elegans, CeHSP17, enables E. coli cells to grow at 50 degrees C, which is their highest growth temperature ever reported. Strikingly, CeHSP17 also rescues the thermal lethality of an E. coli mutant deficient in degP, which encodes a protein quality control factor localized in the periplasmic space. Mechanistically, we show that CeHSP17 is partially localized in the periplasmic space and associated with the inner membrane of E. coli, and it helps to maintain the cell envelope integrity of the E. coli cells at the lethal temperatures. Together, our data indicate that maintaining the cell envelope integrity is crucial for the E. coli cells to grow at high temperatures and also shed new light on the development of thermophilic bacteria for industrial application.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000335909500009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Microbiology; SCI(E); PubMed; 5; ARTICLE; fuxinmiao@pku.edu.cn; changzy@pku.edu.cn; 11; 2004-2011; 196
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/342443]
专题	生命科学学院
推荐引用方式 GB/T 7714	Ezemaduka, Anastasia N.,Yu, Jiayu,Shi, Xiaodong,et al. A Small Heat Shock Protein Enables Escherichia coli To Grow at a Lethal Temperature of 50 degrees C Conceivably by Maintaining Cell Envelope Integrity[J]. journal of bacteriology,2014.
APA	Ezemaduka, Anastasia N..,Yu, Jiayu.,Shi, Xiaodong.,Zhang, Kaiming.,Yin, Chang-Cheng.,...&Chang, Zengyi.(2014).A Small Heat Shock Protein Enables Escherichia coli To Grow at a Lethal Temperature of 50 degrees C Conceivably by Maintaining Cell Envelope Integrity.journal of bacteriology.
MLA	Ezemaduka, Anastasia N.,et al."A Small Heat Shock Protein Enables Escherichia coli To Grow at a Lethal Temperature of 50 degrees C Conceivably by Maintaining Cell Envelope Integrity".journal of bacteriology (2014).