Systematically labeling developmental stage-specific genes for the study   of pancreatic beta-cell differentiation from human embryonic stem cells

doi:10.1038/cr.2014.118

CORC > 北京大学 > 生命科学学院

	Systematically labeling developmental stage-specific genes for the study of pancreatic beta-cell differentiation from human embryonic stem cells
	Liu, Haisong ; Yang, Huan ; Zhu, Dicong ; Sui, Xin ; Li, Juan ; Liang, Zhen ; Xu, Lei ; Chen, Zeyu ; Yao, Anzhi ; Zhang, Long ; Zhang, Xi ; Yi, Xing ; Liu, Meng ; Xu, Shiqing ; Zhang, Wenjian ; Lin, Hua ; Xie, Lan ; Lou, Jinning ; Zhang, Yong ; Xi, Jianzhong ; Deng, Hongkui
刊名	细胞研究英文版
	2014
关键词	gene labeling pancreatic beta cell directed differentiation embryonic stem cell SUSD2 INSULIN-PRODUCING CELLS IN-VITRO REGENERATIVE MEDICINE EXPRESSION NEUROGENIN3 PROGENITORS ENDODERM GENERATION DERIVATION LINEAGE
DOI	10.1038/cr.2014.118
英文摘要	The applications of human pluripotent stem cell (hPSC)-derived cells in regenerative medicine has encountered a long-standing challenge: how can we efficiently obtain mature cell types from hPSCs? Attempts to address this problem are hindered by the complexity of controlling cell fate commitment and the lack of sufficient developmental knowledge for guiding hPSC differentiation. Here, we developed a systematic strategy to study hPSC differentiation by labeling sequential developmental genes to encompass the major developmental stages, using the directed differentiation of pancreatic beta cells from hPSCs as a model. We therefore generated a large panel of pancreas-specific mono-and dual-reporter cell lines. With this unique platform, we visualized the kinetics of the entire differentiation process in real time for the first time by monitoring the expression dynamics of the reporter genes, identified desired cell populations at each differentiation stage and demonstrated the ability to isolate these cell populations for further characterization. We further revealed the expression profiles of isolated NGN3-eGFP(+) cells by RNA sequencing and identified sushi domain-containing 2 (SUSD2) as a novel surface protein that enriches for pancreatic endocrine progenitors and early endocrine cells both in human embryonic stem cells (hESC)-derived pancreatic cells and in the developing human pancreas. Moreover, we captured a series of cell fate transition events in real time, identified multiple cell subpopulations and unveiled their distinct gene expression profiles, among heterogeneous progenitors for the first time using our dual reporter hESC lines. The exploration of this platform and our new findings will pave the way to obtain mature beta cells in vitro.; Cell Biology; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 2; ARTICLE; xi@coe.pku.edu.cn; hongkui_deng@pku.edu.cn; 10; 1181-1200; 24
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/342240]
专题	生命科学学院工学院
推荐引用方式 GB/T 7714	Liu, Haisong,Yang, Huan,Zhu, Dicong,et al. Systematically labeling developmental stage-specific genes for the study of pancreatic beta-cell differentiation from human embryonic stem cells[J]. 细胞研究英文版,2014.
APA	Liu, Haisong.,Yang, Huan.,Zhu, Dicong.,Sui, Xin.,Li, Juan.,...&Deng, Hongkui.(2014).Systematically labeling developmental stage-specific genes for the study of pancreatic beta-cell differentiation from human embryonic stem cells.细胞研究英文版.
MLA	Liu, Haisong,et al."Systematically labeling developmental stage-specific genes for the study of pancreatic beta-cell differentiation from human embryonic stem cells".细胞研究英文版 (2014).