HPV16 early gene E5 specifically reduces miRNA-196a in cervical cancer   cells

doi:10.1038/srep07653

CORC > 北京大学 > 生命科学学院

	HPV16 early gene E5 specifically reduces miRNA-196a in cervical cancer cells
	Liu, Chanzhen ; Lin, Jianfei ; Li, Lianqin ; Zhang, Yonggang ; Chen, Weiling ; Cao, Zeyi ; Zuo, Huancong ; Chen, Chunling ; Kee, Kehkooi
刊名	scientific reports
	2015
关键词	HUMAN-PAPILLOMAVIRUS TYPE-16 MICRORNA EXPRESSION LUNG-CANCER CARCINOMA CELLS E6 ONCOPROTEIN MESSENGER-RNA PROTEIN PROLIFERATION P53 CARCINOGENESIS
DOI	10.1038/srep07653
英文摘要	High-risk human papillomavirus (HPV) type 16, which is responsible for greater than 50% of cervical cancer cases, is the most prevalent and lethal HPV type. However, the molecular mechanisms of cervical carcinogenesis remain elusive, particularly the early steps of HPV infection that may transform normal cervical epithelium into a pre-neoplastic state. Here, we report that a group of microRNAs (microRNAs) were aberrantly decreased in HPV16-positive normal cervical tissues, and these groups of microRNAs are further reduced in cervical carcinoma. Among these miRNAs, miR196a expression is the most reduced in HPV16-infected tissues. Interestingly, miR196a expression is low in HPV16-positive cervical cancer cell lines but high in HPV16-negative cervical cancer cell lines. Furthermore, we found that only HPV16 early gene E5 specifically down-regulated miRNA196a in the cervical cancer cell lines. In addition, HoxB8, a known miR196a target gene, is up-regulated in the HPV16 cervical carcinoma cell line but not in HPV18 cervical cancer cell lines. Various doses of miR196a affected cervical cancer cell proliferation and apoptosis. Altogether, these results suggested that HPV16 E5 specifically down-regulates miR196a upon infection of the human cervix and initiates the transformation of normal cervix cells to cervical carcinoma.; Multidisciplinary Sciences; SCI(E); PubMed; 0; ARTICLE; lynnchan9@126.com; kkee@tsinghua.edu.cn; 7653; 5
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/341704]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu, Chanzhen,Lin, Jianfei,Li, Lianqin,et al. HPV16 early gene E5 specifically reduces miRNA-196a in cervical cancer cells[J]. scientific reports,2015.
APA	Liu, Chanzhen.,Lin, Jianfei.,Li, Lianqin.,Zhang, Yonggang.,Chen, Weiling.,...&Kee, Kehkooi.(2015).HPV16 early gene E5 specifically reduces miRNA-196a in cervical cancer cells.scientific reports.
MLA	Liu, Chanzhen,et al."HPV16 early gene E5 specifically reduces miRNA-196a in cervical cancer cells".scientific reports (2015).