miR-290/371-Mbd2-Myc circuit regulates glycolytic metabolism to promote pluripotency

doi:10.15252/embj.201490441

CORC > 北京大学 > 生命科学学院

	miR-290/371-Mbd2-Myc circuit regulates glycolytic metabolism to promote pluripotency
	Cao Yang ; Guo Wen-Ting ; Tian Shengya ; He Xiaoping ; Wang Xi-Wen ; Liu Xiaomeng ; Gu Kai-Li ; Ma Xiaoyu ; Huang De ; Hu Lan ; Cai Yongping ; Zhang Huafeng ; Wang Yangming ; Gao Ping
刊名	the embo journal
	2015
关键词	Mbd2,glycolysis,metabolism,microRNA,pluripotency
DOI	10.15252/embj.201490441
英文摘要	Enhanced glycolysis is a main feature of pluripotent stem cells (PSCs) and is proposed to be important for the maintenance and induction of pluripotency. The molecular mechanism underlying enhanced glycolysis in PSCs is not clear. Using Dgcr8-/- mouse embryonic stem cells (ESCs) that lack mature miRNAs, we found that miR-290 cluster of miRNAs stimulates glycolysis by upregulating glycolytic enzymes Pkm2 and Ldha, which are also essential for the induction of pluripotency during reprogramming. Mechanistically, we identified Mbd2, a reader for methylated CpGs, as the target of miR-290 cluster that represses glycolysis and reprogramming. Furthermore, we discovered Myc as a key target of Mbd2 that controls metabolic switch in ESCs. Importantly, we demonstrated that miR-371 cluster, a human homolog of miR-290 cluster, stimulates glycolysis to promote the reprogramming of human fibroblasts. Hence, we identified a previously unappreciated mechanism by which miR-290/371 miRNAs orchestrate epigenetic, transcriptional and metabolic networks to promote pluripotency in PSCs and during reprogramming.? 2015 The Authors.; SCI(E); PubMed; 0; ARTICLE; yangming.wang@pku.edu.cn; pgao2@ustc.edu.cn; 5; 609-23; 34
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/341536]
专题	生命科学学院
推荐引用方式 GB/T 7714	Cao Yang,Guo Wen-Ting,Tian Shengya,et al. miR-290/371-Mbd2-Myc circuit regulates glycolytic metabolism to promote pluripotency[J]. the embo journal,2015.
APA	Cao Yang.,Guo Wen-Ting.,Tian Shengya.,He Xiaoping.,Wang Xi-Wen.,...&Gao Ping.(2015).miR-290/371-Mbd2-Myc circuit regulates glycolytic metabolism to promote pluripotency.the embo journal.
MLA	Cao Yang,et al."miR-290/371-Mbd2-Myc circuit regulates glycolytic metabolism to promote pluripotency".the embo journal (2015).