Arabidopsis DET1 degrades HFR1 but stabilizes PIF1 to precisely regulate   seed germination

doi:10.1073/pnas.1502405112

CORC > 北京大学 > 生命科学学院

	Arabidopsis DET1 degrades HFR1 but stabilizes PIF1 to precisely regulate seed germination
	Shi, Hui ; Wang, Xin ; Mo, Xiaorong ; Tang, Chao ; Zhong, Shangwei ; Deng, Xing Wang
刊名	proceedings of the national academy of sciences of the united states of america
	2015
关键词	seed germination DET1-COP10 CDD-CUL4 HFR1-PIF1 phyB NEGATIVE REGULATOR PHYTOCHROME-B LIGHT DEGRADATION THALIANA PHOTOMORPHOGENESIS SEEDLINGS PHOSPHORYLATION DORMANCY PROTEIN
DOI	10.1073/pnas.1502405112
英文摘要	Seed is an essential propagation organ and a critical strategy adopted by terrestrial flowering plants to colonize the land. The ability of seeds to accurately respond to light is vital for plant survival. However, the underlying mechanism is largely unknown. In this study, we reveal a circuit of triple feed-forward loops adopted by Arabidopsis seeds to exclusively repress germination in dark conditions and precisely initiate germination under diverse light conditions. We identify that de-etiolated 1 (DET1), an evolutionarily conserved protein, is a central repressor of light-induced seed germination. Genetic analysis demonstrates that DET1 functions upstream of long hypocotyl in far-red 1 (HFR1) and phytochrome interacting factor 1 (PIF1), the key positive and negative transcription regulators in seed germination. We further find that DET1 and constitutive photomorphogenic 10 (COP10) target HFR1 for protein degradation by assembling a COP10-DET1-damaged DNA binding protein 1-cullin4 E3 ligase complex. Moreover, DET1 and COP10 directly interact with and promote the protein stability of PIF1. Computational modeling reveals that phytochrome B (phyB)-DET1-HFR1- PIF1 and phyB-DET1-Protease-PIF1 are new signaling pathways, independent of the previously identified phyB-PIF1 pathway, respectively mediating the rapid and time-lapse responses to light irradiation. The model-simulated results are highly consistent with their experimental validations, suggesting that our mathematical model captures the essence of Arabidopsis seed germination networks. Taken together, this study provides a comprehensive molecular framework for light-regulated seed germination, improving our understanding of how plants respond to changeable environments.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000351477000063&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); PubMed; 13; ARTICLE; shangwei.zhong@pku.edu.cn; xingwang.deng@yale.edu; 12; 3817-3822; 112
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/341399]
专题	生命科学学院
推荐引用方式 GB/T 7714	Shi, Hui,Wang, Xin,Mo, Xiaorong,et al. Arabidopsis DET1 degrades HFR1 but stabilizes PIF1 to precisely regulate seed germination[J]. proceedings of the national academy of sciences of the united states of america,2015.
APA	Shi, Hui,Wang, Xin,Mo, Xiaorong,Tang, Chao,Zhong, Shangwei,&Deng, Xing Wang.(2015).Arabidopsis DET1 degrades HFR1 but stabilizes PIF1 to precisely regulate seed germination.proceedings of the national academy of sciences of the united states of america.
MLA	Shi, Hui,et al."Arabidopsis DET1 degrades HFR1 but stabilizes PIF1 to precisely regulate seed germination".proceedings of the national academy of sciences of the united states of america (2015).