Structural Insight into the Binding of Hypoxanthine with Purine   Nucleoside Phosphorylase from Streptococcus mutants

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	Structural Insight into the Binding of Hypoxanthine with Purine Nucleoside Phosphorylase from Streptococcus mutants
	Hou Qiao-Ming ; Yang Na ; Liu Xiang ; Wang Hong-Fei ; Li Lan-Fen ; Su Xiao-Dong
刊名	结构化学
	2011
关键词	Streptococcus mutants purine nucleoside phosphorylase crystal structure TRANSITION-STATE ANALOGS CRYSTAL-STRUCTURE CALF SPLEEN RESOLUTION MECHANISM SEQUENCE ENZYME ION
英文摘要	Purine nucleoside phosphorylase is a key enzyme in the purine-salvage pathway and an attractive target for drug design. The crystal structure of Streptococcus mutants purine nucleoside phosphorylase (Smu PNP) has been solved by molecular replacement at 1.80 angstrom resolution and refined to R factors of 19.9%/23.7% (R-cryst/R-free). Sequence alignment and structural comparison show that Smu PNP has more similarity with PNPs isolated from human and malarial sources than the bacterial PNPs. The structure complexed with hypoxanthine (HPA) and sulfate ion was solved at 2.24 angstrom resolution and refined to R factors of 21.6%/24.1% (R-cryst/R-free). It is interesting to note that the resulting electron density indicated the product, HPA, presents in the active site although inosine was included in the crystallization mixture with Smu PNP. Asn233 and Glu191 are the important residues for ligand binding and recognition. Comparison with PNPs from different species gives detailed information about binding of small molecules on the active site, which is important for the studies of enzymatic mechanism and rational design of specific inhibitors for PNPs.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000287112000007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Inorganic & Nuclear; Crystallography; SCI(E); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 0; ARTICLE; 1; 43-50; 30
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/320223]
专题	生命科学学院
推荐引用方式 GB/T 7714	Hou Qiao-Ming,Yang Na,Liu Xiang,et al. Structural Insight into the Binding of Hypoxanthine with Purine Nucleoside Phosphorylase from Streptococcus mutants[J]. 结构化学,2011.
APA	Hou Qiao-Ming,Yang Na,Liu Xiang,Wang Hong-Fei,Li Lan-Fen,&Su Xiao-Dong.(2011).Structural Insight into the Binding of Hypoxanthine with Purine Nucleoside Phosphorylase from Streptococcus mutants.结构化学.
MLA	Hou Qiao-Ming,et al."Structural Insight into the Binding of Hypoxanthine with Purine Nucleoside Phosphorylase from Streptococcus mutants".结构化学 (2011).