拟南芥细胞中存在中间纤维的研究

CORC > 北京大学 > 生命科学学院

	拟南芥细胞中存在中间纤维的研究; The existence of intermediate filament in Arabidopsis thaliana cell
	赵大中 ; 陈丹英 ; 陈敏 ; 刘春香 ; 杨澄 ; 翟中和
刊名	植物学报
	1999
关键词	拟南芥中间纤维选择性抽提免疫印迹反应体外组装基因克隆 Arabidopsis thaliana intermediate filament selective extraction Western blot assembly in vitro gene cloning
英文摘要	利用整装电镜制样与选择性抽提技术，在拟南芥（Ａｒａｂｉｄｏｐｓｉｓｔｈａｌｉａｎａ（Ｌ．）Ｈｅｙｎｈ）愈伤组织细胞质中观察到直径１０ｎｍ左右的纤维网络结构。免疫印迹分析表明纤维的主要成分是６种多肽，它们分别与动物角蛋白单克隆抗体ＡＥ１、ＡＥ３有免疫交叉反应。利用间接免疫荧光技术，与ＡＥ１和ＡＥ３反应的抗原呈弥散状定位于整个细胞质中，而且１０ｎｍ纤维可以在体外重新组装。以上结果表明，在拟南芥细胞质中存在类角蛋白的中间纤维。以动物中间纤维基因的保守序列为引物，采用ＲＴ＿ＰＣＲ技术，进一步从这一模式植物中克隆到一个ｃＤＮＡ片段，这可能为从分子水平上证明植物中间纤维的存在提供了...; The network structure of cytoplasmic filaments of 10 nm in diameter was detected from callus cells of Arabidopsis thaliana (L.) Heynh by selective extraction combined with whole mount electron microscopy. Western blot analysis showed that the major filament components were 6 polypeptides, which reacted with keratin monoclonal antibody of AE1 or AE3 respectively. By indirect immunofluorescence technique, the AE1 and AE3-reactive antigens were localized throughout the cytoplasm in a diffused pattern. The 10 nm-plant filaments could be reassembled in vitro. These results demonstrated that keratin-like intermediate filaments exist in the cytoplasm of A. thaliana. Using conservative sequence of animal LF genes as primer, a cDNA fragment was further cloned from this model material by RT-PCR, which might shed more light on molecular characterization of IF existence in higher plant.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000082455000002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); 中文核心期刊要目总览(PKU); 中国科学引文数据库(CSCD); 0; 08; 795-799; 41
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/320097]
专题	生命科学学院
推荐引用方式 GB/T 7714	赵大中,陈丹英,陈敏,等. 拟南芥细胞中存在中间纤维的研究, The existence of intermediate filament in Arabidopsis thaliana cell[J]. 植物学报,1999.
APA	赵大中,陈丹英,陈敏,刘春香,杨澄,&翟中和.(1999).拟南芥细胞中存在中间纤维的研究.植物学报.
MLA	赵大中,et al."拟南芥细胞中存在中间纤维的研究".植物学报 (1999).