Highly efficient differentiation of human ES cells and iPS cells into   mature pancreatic insulin-producing cells

doi:10.1038/cr.2009.28

CORC > 北京大学 > 生命科学学院

	Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells
	Zhang, Donghui ; Jiang, Wei ; Liu, Meng ; Sui, Xin ; Yin, Xiaolei ; Chen, Song ; Shi, Yan ; Deng, Hongkui
刊名	细胞研究英文版
	2009
关键词	insulin-producing cell pancreatic differentiation human embryonic stem cells human induced pluripotent cells EMBRYONIC STEM-CELLS HUMAN FIBROBLASTS BETA-CELLS GENERATION MOUSE SOX9
DOI	10.1038/cr.2009.28
英文摘要	Human pluripotent stem cells represent a potentially unlimited source of functional pancreatic endocrine lineage cells. Here we report a highly efficient approach to induce human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells to differentiate into mature insulin-producing cells in a chemical-defined culture system. The differentiated human ES cells obtained by this approach comprised nearly 25% insulin-positive cells as assayed by flow cytometry analysis, which released insulin/C-peptide in response to glucose stimuli in a manner comparable to that of adult human islets. Most of these insulin-producing cells co-expressed mature beta cell-specific markers such as NKX6-1 and PDX1, indicating a similar gene expression pattern to adult islet beta cells in vivo. In this study, we also demonstrated that EGF facilitates the expansion of PDX1-positive pancreatic progenitors. Moreover, our protocol also succeeded in efficiently inducing human iPS cells to differentiate into insulin-producing cells. Therefore, this work not only provides a new model to study the mechanism of human pancreatic specialization and maturation in vitro, but also enhances the possibility of utilizing patient-specific iPS cells for the treatment of diabetes.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000265700100005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Cell Biology; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 242; ARTICLE; 4; 429-438; 19
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/320009]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhang, Donghui,Jiang, Wei,Liu, Meng,et al. Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells[J]. 细胞研究英文版,2009.
APA	Zhang, Donghui.,Jiang, Wei.,Liu, Meng.,Sui, Xin.,Yin, Xiaolei.,...&Deng, Hongkui.(2009).Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells.细胞研究英文版.
MLA	Zhang, Donghui,et al."Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells".细胞研究英文版 (2009).