Crystallization and preliminary X-ray crystallographic analysis of human   PACSIN 1 protein

doi:10.1107/S1744309109049549

CORC > 北京大学 > 生命科学学院

	Crystallization and preliminary X-ray crystallographic analysis of human PACSIN 1 protein
	Bai, Xiaoyun ; Meng, Geng ; Li, Guoming ; Luo, Ming ; Zheng, Xiaofeng
刊名	acta crystallographica section f structural biology and crystallization communications
	2010
关键词	MEMBRANE INVAGINATION SYNDAPIN-I DYNAMIN BAR
DOI	10.1107/S1744309109049549
英文摘要	PACSIN 1, which is mainly detected in brain tissue, is one of the PACSIN-family proteins involved in endocytosis and recruitment of synaptic vesicles. It binds to dynamin, synaptojanin 1 and N-WASP, and functions in vesicle formation and transport. However, the mechanisms of action of PACSIN 1 in these processes are largely unknown. Here, full-length and five C-terminal truncation constructs of human PACSIN 1 have been successfully expressed and purified in Escherichia coli. PACSIN 1 (1-344) was crystallized and diffracted to a resolution of 3.0 angstrom. The crystal belonged to space group C2, with unit-cell parameters a = 158.65, b = 87.38, c = 91.76 angstrom, alpha = 90.00, beta = 113.61, gamma = 90.00 degrees. There were two molecules in the asymmetric unit and the solvent content was estimated to be about 70.47%.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000273072500021&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Crystallography; SCI(E); PubMed; 4; ARTICLE; Pt 1; 73-75; 66
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/319995]
专题	生命科学学院
推荐引用方式 GB/T 7714	Bai, Xiaoyun,Meng, Geng,Li, Guoming,et al. Crystallization and preliminary X-ray crystallographic analysis of human PACSIN 1 protein[J]. acta crystallographica section f structural biology and crystallization communications,2010.
APA	Bai, Xiaoyun,Meng, Geng,Li, Guoming,Luo, Ming,&Zheng, Xiaofeng.(2010).Crystallization and preliminary X-ray crystallographic analysis of human PACSIN 1 protein.acta crystallographica section f structural biology and crystallization communications.
MLA	Bai, Xiaoyun,et al."Crystallization and preliminary X-ray crystallographic analysis of human PACSIN 1 protein".acta crystallographica section f structural biology and crystallization communications (2010).