C-elegans Rab GTPase activating protein TBC-2 promotes cell corpse   degradation by regulating the small GTPase RAB-5

doi:10.1242/dev.035949

CORC > 北京大学 > 生命科学学院

	C-elegans Rab GTPase activating protein TBC-2 promotes cell corpse degradation by regulating the small GTPase RAB-5
	Li, Weida ; Zou, Wei ; Zhao, Dongfeng ; Yan, Jiacong ; Zhu, Zuoyan ; Lu, Jing ; Wang, Xiaochen
刊名	development
	2009
关键词	C. elegans TBC-2 RAB-5 GAP Phagosome maturation Cell corpse degradation PHAGOSOME MATURATION CAENORHABDITIS-ELEGANS APOPTOTIC CELLS PHOSPHATIDYLINOSITOL 3-KINASE DOMAIN MACROPHAGES ENGULFMENT FUSION GAP ACQUISITION
DOI	10.1242/dev.035949
英文摘要	During apoptosis, dying cells are quickly internalized by neighboring cells or phagocytes, and are enclosed in phagosomes that undergo a maturation process to generate the phagoslysosome, in which cell corpses are eventually degraded. It is not well understood how apoptotic cell degradation is regulated. Here we report the identification and characterization of the C. elegans tbc-2 gene, which is required for the efficient degradation of cell corpses. tbc-2 encodes a Rab GTPase activating protein (GAP) and its loss of function affects several events of phagosome maturation, including RAB-5 release, phosphatidylinositol 3-phosphate dynamics, phagosomal acidification, RAB-7 recruitment and lysosome incorporation, which leads to many persistent cell corpses at various developmental stages. Intriguingly, the persistent cell corpse phenotype of tbc-2 mutants can be suppressed by reducing gene expression of rab-5, and overexpression of a GTP-locked RAB-5 caused similar defects in phagosome maturation and cell corpse degradation. We propose that TBC-2 functions as a GAP to cycle RAB-5 from an active GTP-bound to an inactive GDP-bound state, which is required for maintaining RAB-5 dynamics on phagosomes and serves as a switch for the progression of phagosome maturation.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000267218500014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Developmental Biology; SCI(E); PubMed; 23; ARTICLE; 14; 2445-2455; 136
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/319986]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, Weida,Zou, Wei,Zhao, Dongfeng,et al. C-elegans Rab GTPase activating protein TBC-2 promotes cell corpse degradation by regulating the small GTPase RAB-5[J]. development,2009.
APA	Li, Weida.,Zou, Wei.,Zhao, Dongfeng.,Yan, Jiacong.,Zhu, Zuoyan.,...&Wang, Xiaochen.(2009).C-elegans Rab GTPase activating protein TBC-2 promotes cell corpse degradation by regulating the small GTPase RAB-5.development.
MLA	Li, Weida,et al."C-elegans Rab GTPase activating protein TBC-2 promotes cell corpse degradation by regulating the small GTPase RAB-5".development (2009).