Isolation of HAG1 and its regulation by plant hormones during in vitro   floral organogenesis in Hyacinthus orientalis L.

doi:10.1007/s00425-002-0796-3

CORC > 北京大学 > 生命科学学院

	Isolation of HAG1 and its regulation by plant hormones during in vitro floral organogenesis in Hyacinthus orientalis L.
	Li, QZ ; Li, XG ; Bai, SN ; Lu, WL ; Zhang, XS
刊名	planta
	2002
关键词	cytokinin auxin floral organ HAG1 Hyacinthus regeneration (floral bud) REGENERATED FLOWER BUDS HOMEOTIC GENE MERISTEM IDENTITY ORGAN IDENTITY ARABIDOPSIS INDUCTION IMPATIENS APETALA2
DOI	10.1007/s00425-002-0796-3
英文摘要	Floral organs have been successfully induced from the regenerated floral buds of Hyacinthus orientalis L. by precisely controlling exogenous hormones in the medium. Under high concentrations of cytokinin and auxin, the regenerated floral bud produces only tepals. However, at reduced levels of the hormones, the regenerated floral bud can produce stamens and/or carpels with ovules. To understand the molecular mechanism of hormone-regulated flower development, a MADS-box gene, HAG1, which is homologous to AGAMOUS (AG) in Arabidopsis, was isolated from the floral tissues of Hyacinthus. Overexpression of HAG1 in Arabidopsis created flower phenotypes resembling those of the apetala2 mutant and AG transgenic Arabidopsis plants. Furthermore, the HAG1 expression pattern was similar to that of AG, confirming that HAG1 is the ortholog of AG in IIJ,achithus. HAGI rnRNA was first detected in cultured explants at day 5 in the medium containing high levels of cytokinin and auxin, which could induce floral regeneration in vitro. However, no HAG1 mRNA was detected in the cultured explants until day 10 in media with low or no hormones. Further, HAG1 mRNA was detected in the stamens and carpels of regenerated floral buds, but not in the tepals. Our data support the hypothesis that hormone-regulated HAG1 activity is required for the induction of floral buds and the determination of floral organ types during the regeneration of floral buds.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000177757100002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Plant Sciences; SCI(E); EI; 31; ARTICLE; 4; 533-540; 215
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/258009]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, QZ,Li, XG,Bai, SN,et al. Isolation of HAG1 and its regulation by plant hormones during in vitro floral organogenesis in Hyacinthus orientalis L.[J]. planta,2002.
APA	Li, QZ,Li, XG,Bai, SN,Lu, WL,&Zhang, XS.(2002).Isolation of HAG1 and its regulation by plant hormones during in vitro floral organogenesis in Hyacinthus orientalis L..planta.
MLA	Li, QZ,et al."Isolation of HAG1 and its regulation by plant hormones during in vitro floral organogenesis in Hyacinthus orientalis L.".planta (2002).