The sense and antisense expression of gibberellin 20-oxidase gene (rga5)   in rice and its effects on GA(1) level and agronomic traits

CORC > 北京大学 > 生命科学学院

	The sense and antisense expression of gibberellin 20-oxidase gene (rga5) in rice and its effects on GA(1) level and agronomic traits
	Yan, YX ; An, CG ; Li, L ; Gu, JY ; Wang, LJ ; Mi, SL ; Chen, ZL
刊名	科学通报英文版
	2003
关键词	Oryza sativa gibberellin 20-oxidase sense antisense transgenic ARABIDOPSIS-THALIANA ENCODES CLONING LOCUS BIOSYNTHESIS PROTEIN GROWTH
英文摘要	A gibberellin 20-oxidase gene rga5 was isolated by PCR from genomic DNA of rice (Oryza sativa ssp indica) cultivars 'Aizizhan' and 'Nante'. Compared with the reported OsGA20ox, the rga5 was partial-frame-shifted with 11 different amino acids. Then the rga5 with CaMV 35S promotor and NOS terminator was inserted into the poly-linker site of pCambia1301 to construct sense and antisense gene expressing vectors pSrga5 and pArga5. The transgenic plants were obtained by biolistic bombardment with pSrga5 or pArga5. The transgenic rice plants showed that the overexpression and antisense-expression of rga5 have remarkable effects on the biological characters of rice. The sense transgenic plants showed heightening with longer spike, more seed-bearing and unaffected flowering, whereas antisense transgenic plants showed dwarfing, early-flowering with slender stem, dark leaf color, shorter leaf and shorter spike. The PCR amplification and Southern blot hybridization showed that the rga5 has been integrated into the transgenic rice genome and the transcription of rga5 was identified by Northern blot hybridization. In the sense transgenic plants the GA(1) content increased of about 50%, however, the antisense transgenic rice decreased of 90% approximately compared with control plant 'Zhonghua 8'. These results demonstrated that the rga5 is a functional gene encoding gibberellin 20-oxidase in rice. Over-expressing rga5 significantly increases the endogenous GA(1) level and plant height of rice, whereas the expression of antisense rga5 decreases the GA(1) level and plant height of rice dramatically.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000181790200008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); 1; ARTICLE; 5; 443-448; 48
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/256052]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yan, YX,An, CG,Li, L,et al. The sense and antisense expression of gibberellin 20-oxidase gene (rga5) in rice and its effects on GA(1) level and agronomic traits[J]. 科学通报英文版,2003.
APA	Yan, YX.,An, CG.,Li, L.,Gu, JY.,Wang, LJ.,...&Chen, ZL.(2003).The sense and antisense expression of gibberellin 20-oxidase gene (rga5) in rice and its effects on GA(1) level and agronomic traits.科学通报英文版.
MLA	Yan, YX,et al."The sense and antisense expression of gibberellin 20-oxidase gene (rga5) in rice and its effects on GA(1) level and agronomic traits".科学通报英文版 (2003).