Immunolocalization of arabinogalactan proteins and pectins in floral   buds of cucumber (Cucumis sativus L.) during sex determination

doi:10.1111/j.1744-7909.2005.00023.x

CORC > 北京大学 > 生命科学学院

	Immunolocalization of arabinogalactan proteins and pectins in floral buds of cucumber (Cucumis sativus L.) during sex determination
	Peng, YB ; Zou, C ; Gong, HQ ; Bai, SN ; Xu, ZH ; Li, YQ
刊名	journal of integrative plant biology
	2005
关键词	arabinogalactan proteins cucumber (Cucumis sativus L.) floral buds immunolocalization pectins NICOTIANA-TABACUM-L CELL-WALLS IMMUNOGOLD LOCALIZATION ARABIDOPSIS-THALIANA SPATIAL REGULATION POLLEN TUBES POLYSACCHARIDES EXPRESSION PEA METHYLESTERASE
DOI	10.1111/j.1744-7909.2005.00023.x
英文摘要	Arabinogalactan proteins (AGPs) and pectins were detected in the floral buds of cucumber (Cucumis sativus L.) during its sex determination using the following monoclonal antibodies: MAC 207 (recognizes AGP epitopes); JIM 8 (recognizes a subset of AGP epitopes); and JIM 5 and JIM 7 (epitopes of pectins esterified to various degrees). In the stem apex meristem (SAM) of the cucumber, epitopes of MAC 207, JIM 7, and JIM 5 were localized in the cells from second to third peripheral layers when the sex organ primodiurn began to differentiate; epitopes of MAC 207 and JIM 5 were also detected in the ragged edge cells. A very dense labeling signal with MAC 207 was observed in the carpel and pistil primodium. The AGP epitopes recognized by JIM 8 were localized in the anther of the male flower and the anther-like portion of the stagnant stamen of the female flower. This suggests that the AGPs and pectins in the SAM of the cucumber are closely associated with the differentiation of the SAM, from meristernatic cells to floral primodium. The subset of AGPs recognized by JIM 8 may play an important role in stamen formation.; Biochemistry & Molecular Biology; Plant Sciences; SCI(E); 中国科学引文数据库(CSCD); 2; ARTICLE; 2; 194-200; 47
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/254478]
专题	生命科学学院
推荐引用方式 GB/T 7714	Peng, YB,Zou, C,Gong, HQ,et al. Immunolocalization of arabinogalactan proteins and pectins in floral buds of cucumber (Cucumis sativus L.) during sex determination[J]. journal of integrative plant biology,2005.
APA	Peng, YB,Zou, C,Gong, HQ,Bai, SN,Xu, ZH,&Li, YQ.(2005).Immunolocalization of arabinogalactan proteins and pectins in floral buds of cucumber (Cucumis sativus L.) during sex determination.journal of integrative plant biology.
MLA	Peng, YB,et al."Immunolocalization of arabinogalactan proteins and pectins in floral buds of cucumber (Cucumis sativus L.) during sex determination".journal of integrative plant biology (2005).